| pubmed-article:2497807 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2497807 | lifeskim:mentions | umls-concept:C1135183 | lld:lifeskim |
| pubmed-article:2497807 | lifeskim:mentions | umls-concept:C0016431 | lld:lifeskim |
| pubmed-article:2497807 | lifeskim:mentions | umls-concept:C0017953 | lld:lifeskim |
| pubmed-article:2497807 | lifeskim:mentions | umls-concept:C0043100 | lld:lifeskim |
| pubmed-article:2497807 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
| pubmed-article:2497807 | lifeskim:mentions | umls-concept:C0040287 | lld:lifeskim |
| pubmed-article:2497807 | lifeskim:mentions | umls-concept:C1264633 | lld:lifeskim |
| pubmed-article:2497807 | lifeskim:mentions | umls-concept:C0220927 | lld:lifeskim |
| pubmed-article:2497807 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2497807 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2497807 | pubmed:dateCreated | 1989-7-6 | lld:pubmed |
| pubmed-article:2497807 | pubmed:abstractText | We have used dansyl chloride (5-dimethylamino-1-naphthalenesulfonyl choloride) to form dansyl derivatives of amine-containing compounds in follicular fluid or highly purified fractions containing a low molecular weight (MW) inhibitor of follicle-stimulating hormone (FSH) binding to receptor (FSH-BI). This approach allowed sensitive detection of the derivatives based on their fluorescent properties. By taking advantage of the hydrophobic nature of the dansyl group, a dansyl derivative (RF = 0.15) identified in low MW FSH-BI preparations was purified from porcine follicular fluid. Based on chromatographic criteria using four different systems (thin-layer chromatography [TLC] and high performance liquid chromatography), the derivatized factor (D15) that was purified appeared to be homogeneous. A direct, chemical assay was developed for quantification of D15 from follicular fluid or tissue extracts. The highest concentration (153 ng/mg) of D15 was found in ovarian tissue of adult rats, lesser amounts were observed in kidney and liver tissues (93 and 62 ng/mg, respectively) and even less in diaphram and heart tissues (5 and 0.5 ng/mg, respectively). High concentrations of D15 were observed in derivatized extracts of tests from immature rats in which approximately twice as much D15 was found in Leydig cells (241 ng/mg) as in seminiferous tubules (136 ng/mg). In porcine ovarian tissue, granulosa cells from large follicles and corpora lutea (69 and 91 ng/mg, respectively) contained at least 4-fold higher concentrations than follicle wall tissue (14 ng/ml). Relative concentrations of D15 material were also determined in pools of bovine follicular fluid previously shown to contain low MW FSH-BI.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:2497807 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2497807 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2497807 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2497807 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2497807 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2497807 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2497807 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2497807 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2497807 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2497807 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2497807 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:2497807 | pubmed:issn | 0006-3363 | lld:pubmed |
| pubmed-article:2497807 | pubmed:author | pubmed-author:FordJ JJJ | lld:pubmed |
| pubmed-article:2497807 | pubmed:author | pubmed-author:ReichertL... | lld:pubmed |
| pubmed-article:2497807 | pubmed:author | pubmed-author:SlussP MPM | lld:pubmed |
| pubmed-article:2497807 | pubmed:author | pubmed-author:KrishnanK AKA | lld:pubmed |
| pubmed-article:2497807 | pubmed:author | pubmed-author:BrancaA AAA | lld:pubmed |
| pubmed-article:2497807 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2497807 | pubmed:volume | 40 | lld:pubmed |
| pubmed-article:2497807 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2497807 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2497807 | pubmed:pagination | 407-15 | lld:pubmed |
| pubmed-article:2497807 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2497807 | pubmed:meshHeading | pubmed-meshheading:2497807-... | lld:pubmed |
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| pubmed-article:2497807 | pubmed:meshHeading | pubmed-meshheading:2497807-... | lld:pubmed |
| pubmed-article:2497807 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2497807 | pubmed:articleTitle | Purification, measurement, and tissue distribution of a dansyl-derivatized glycopeptide from low-molecular weight follicle-stimulating hormone-inhibitor-containing fractions of porcine follicular fluid. | lld:pubmed |
| pubmed-article:2497807 | pubmed:affiliation | Department of Biochemistry, Albany Medical College, New York 12208. | lld:pubmed |
| pubmed-article:2497807 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2497807 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2497807 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:2497807 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
