| pubmed-article:2481191 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2481191 | lifeskim:mentions | umls-concept:C1882687 | lld:lifeskim |
| pubmed-article:2481191 | lifeskim:mentions | umls-concept:C0034414 | lld:lifeskim |
| pubmed-article:2481191 | lifeskim:mentions | umls-concept:C1515926 | lld:lifeskim |
| pubmed-article:2481191 | lifeskim:mentions | umls-concept:C1450468 | lld:lifeskim |
| pubmed-article:2481191 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2481191 | pubmed:dateCreated | 1990-2-6 | lld:pubmed |
| pubmed-article:2481191 | pubmed:abstractText | To elucidate the molecular mechanism underlying the adverse depression of myocardial contractility observed during antiarrhythmic therapy of quinidine, we investigated its action on the phosphatidylethanolamine N-methyltransferase (EC 2.1.1.17) activities of cardiac subcellular membranes. Rat heart sarcolemma, mitochondria, and microsomes (sarcoplasmic reticular fragments) were isolated, and the three catalytic sites for N-methylation activities were examined with 0.055 (site I), 10 (site II), and 150 (site III) microM concentrations of S-adenosyl-L-[methyl-3H]methionine as a methyl donor. Total methyl group incorporation into sarcolemmal phosphatidylethanolamine was depressed by 10(-6)-10(-3) M quinidine at sites II and III. The activity of site I was stimulated at low (10(-9) M) concentrations and inhibited at high concentrations of the drug. A similar behaviour was observed with procainamide, although the inhibitory effect was less pronounced and was not additive with quinidine. Quinidine-induced inhibition was associated with a depression of Vmax, while the apparent affinity for S-adenosyl-L-methionine was unaltered. Analysis of individual methylated phospholipids confirmed inhibition by quinidine at sites II and III in sarcolemma. Microsomal phosphatidylethanolamine N-methylation was affected by 10(-6) M quinidine only at site II, whereas no changes were noted in mitochondria. Quinidine also inhibited both the positive inotropic response and concomitant increase in tissue N-methylated phospholipids observed upon L-methionine perfusion of rat heart. These results suggest that quinidine alters the intramembranal level of N-methylated phospholipids, and this may serve as a biochemical mechanism contributing to its negative inotropic effect. | lld:pubmed |
| pubmed-article:2481191 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2481191 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2481191 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2481191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2481191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2481191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2481191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2481191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2481191 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2481191 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:2481191 | pubmed:issn | 0160-2446 | lld:pubmed |
| pubmed-article:2481191 | pubmed:author | pubmed-author:DhallaN SNS | lld:pubmed |
| pubmed-article:2481191 | pubmed:author | pubmed-author:TairaYY | lld:pubmed |
| pubmed-article:2481191 | pubmed:author | pubmed-author:GuptaM PMP | lld:pubmed |
| pubmed-article:2481191 | pubmed:author | pubmed-author:GangulyP KPK | lld:pubmed |
| pubmed-article:2481191 | pubmed:author | pubmed-author:PanagiaVV | lld:pubmed |
| pubmed-article:2481191 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2481191 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:2481191 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2481191 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2481191 | pubmed:pagination | 763-9 | lld:pubmed |
| pubmed-article:2481191 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2481191 | pubmed:meshHeading | pubmed-meshheading:2481191-... | lld:pubmed |
| pubmed-article:2481191 | pubmed:meshHeading | pubmed-meshheading:2481191-... | lld:pubmed |
| pubmed-article:2481191 | pubmed:meshHeading | pubmed-meshheading:2481191-... | lld:pubmed |
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| pubmed-article:2481191 | pubmed:meshHeading | pubmed-meshheading:2481191-... | lld:pubmed |
| pubmed-article:2481191 | pubmed:meshHeading | pubmed-meshheading:2481191-... | lld:pubmed |
| pubmed-article:2481191 | pubmed:meshHeading | pubmed-meshheading:2481191-... | lld:pubmed |
| pubmed-article:2481191 | pubmed:meshHeading | pubmed-meshheading:2481191-... | lld:pubmed |
| pubmed-article:2481191 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2481191 | pubmed:articleTitle | Alterations of phosphatidylethanolamine N-methylation in rat heart by quinidine. | lld:pubmed |
| pubmed-article:2481191 | pubmed:affiliation | Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Winnipeg, Canada. | lld:pubmed |
| pubmed-article:2481191 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2481191 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:2481191 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2481191 | lld:pubmed |
