| pubmed-article:2481034 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2481034 | lifeskim:mentions | umls-concept:C0006644 | lld:lifeskim |
| pubmed-article:2481034 | lifeskim:mentions | umls-concept:C0027882 | lld:lifeskim |
| pubmed-article:2481034 | lifeskim:mentions | umls-concept:C0001721 | lld:lifeskim |
| pubmed-article:2481034 | lifeskim:mentions | umls-concept:C0521116 | lld:lifeskim |
| pubmed-article:2481034 | pubmed:dateCreated | 1990-1-29 | lld:pubmed |
| pubmed-article:2481034 | pubmed:abstractText | 1. Ionic mechanisms related to the caffeine-induced current (Icaffeine) were examined in the single isolated sympathetic neurones of the bull-frog. We used the 'concentration-jump' technique in combination with intracellular perfusion and a rapid external solution change, under single-electrode voltage-clamp conditions. 2. Icaffeine was pharmacologically separated into a tetraethylammonium (TEA)-sensitive transient outward current (ITO), a picrotoxin (PTX)-sensitive transient inward current (ITI) and a TEA- and PTX-insensitive sustained inward current (ISI). At low concentrations of caffeine, a sustained outward current (ISO) was observed instead of ISI. 3. All components of Icaffeine were abolished by intracellular perfusion of 30 mM-EGTA. Pre-treatment with A23187 or ryanodine or the simultaneous application of procaine either reduced or abolished all the components of Icaffeine in a dose-dependent manner. The concentration causing 50% inhibition (IC50) was 10(-8) M for A23187 and 2 mM for procaine. 4. The peak response of ITO increased abruptly at caffeine concentrations between 3 and 6 mM followed by saturation above 30 mM. A notch was observed on the rising phase of ITO. 5. The reversal potential (Ecaffeine) of ITO shifted 58 mV for a tenfold change of the extracellular K+ concentration. External application of TEA blocked ITO with an IC50 of 1 mM. ITO was relatively insensitive to apamin, 4-aminopyridine and muscarine. 6. In external solution containing 2 mM-Ca2+, ITO induced by 10 mM-caffeine recovered completely within 3 min from a previous exposure to caffeine. In the absence of extracellular Ca2+, there was little such recovery. A 5 min treatment in a Ca2+-free solution reduced ITO induced by the first application of caffeine by 5%. With a continuous application of 3 mM-caffeine, the amplitude of ITO induced by 10 mM-caffeine reduced in 1 min, and showed a partial recovery in 3 min. The amplitude of ITO increased by increasing the concentration of intracellular Cl-. 7. ITI was activated around the peak of ITO and was rapidly inactivated. ITI was evoked at caffeine concentrations of about 6-10 mM. When the intracellular Cl- concentration was changed, the amplitude of ITI behaved like a Cl- electrode. The Ecaffeine of ITI was close to the Cl- equilibrium potential (ECl). 8. ISI was a 'plateau' response and persisted for over 3 min. ISI was due to a decrease in K+ conductance. In the presence of muscarine (3 x 10(-5) M), ISI was occluded.(ABSTRACT TRUNCATED AT 400 WORDS) | lld:pubmed |
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| pubmed-article:2481034 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2481034 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2481034 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2481034 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2481034 | pubmed:month | May | lld:pubmed |
| pubmed-article:2481034 | pubmed:issn | 0022-3751 | lld:pubmed |
| pubmed-article:2481034 | pubmed:author | pubmed-author:AkaikeNN | lld:pubmed |
| pubmed-article:2481034 | pubmed:author | pubmed-author:SadoshimaJJ | lld:pubmed |
| pubmed-article:2481034 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2481034 | pubmed:volume | 412 | lld:pubmed |
| pubmed-article:2481034 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2481034 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2481034 | pubmed:pagination | 221-44 | lld:pubmed |
| pubmed-article:2481034 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:2481034 | pubmed:meshHeading | pubmed-meshheading:2481034-... | lld:pubmed |
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| pubmed-article:2481034 | pubmed:meshHeading | pubmed-meshheading:2481034-... | lld:pubmed |
| pubmed-article:2481034 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2481034 | pubmed:articleTitle | Caffeine affects four different ionic currents in the bull-frog sympathetic neurone. | lld:pubmed |
| pubmed-article:2481034 | pubmed:affiliation | Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan. | lld:pubmed |
| pubmed-article:2481034 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2481034 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:2481034 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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