| pubmed-article:2479296 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2479296 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:2479296 | lifeskim:mentions | umls-concept:C0040284 | lld:lifeskim |
| pubmed-article:2479296 | lifeskim:mentions | umls-concept:C0231441 | lld:lifeskim |
| pubmed-article:2479296 | lifeskim:mentions | umls-concept:C0180454 | lld:lifeskim |
| pubmed-article:2479296 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2479296 | pubmed:dateCreated | 1989-11-27 | lld:pubmed |
| pubmed-article:2479296 | pubmed:abstractText | A method for quantification of anchorage-dependent cells in culture on plane surfaces or on microcarriers is proposed. It is based on Coomassie brilliant blue R-250 adsorption, followed by elution of the dye and measurement by spectrophotometry at 595 nm. A linear correlation (r = 0.988 to 0.996) was observed between absorbance and cell number along a large range of cell densities. This technique may be used for monitoring cell growth, from seeding of initial inoculi to scaling up of cultures in bioreactors. | lld:pubmed |
| pubmed-article:2479296 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2479296 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479296 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2479296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479296 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2479296 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:2479296 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:2479296 | pubmed:author | pubmed-author:BorojevicRR | lld:pubmed |
| pubmed-article:2479296 | pubmed:author | pubmed-author:MargisRR | lld:pubmed |
| pubmed-article:2479296 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2479296 | pubmed:volume | 181 | lld:pubmed |
| pubmed-article:2479296 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2479296 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2479296 | pubmed:pagination | 209-11 | lld:pubmed |
| pubmed-article:2479296 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2479296 | pubmed:meshHeading | pubmed-meshheading:2479296-... | lld:pubmed |
| pubmed-article:2479296 | pubmed:meshHeading | pubmed-meshheading:2479296-... | lld:pubmed |
| pubmed-article:2479296 | pubmed:meshHeading | pubmed-meshheading:2479296-... | lld:pubmed |
| pubmed-article:2479296 | pubmed:meshHeading | pubmed-meshheading:2479296-... | lld:pubmed |
| pubmed-article:2479296 | pubmed:meshHeading | pubmed-meshheading:2479296-... | lld:pubmed |
| pubmed-article:2479296 | pubmed:meshHeading | pubmed-meshheading:2479296-... | lld:pubmed |
| pubmed-article:2479296 | pubmed:meshHeading | pubmed-meshheading:2479296-... | lld:pubmed |
| pubmed-article:2479296 | pubmed:meshHeading | pubmed-meshheading:2479296-... | lld:pubmed |
| pubmed-article:2479296 | pubmed:meshHeading | pubmed-meshheading:2479296-... | lld:pubmed |
| pubmed-article:2479296 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2479296 | pubmed:articleTitle | Quantification of attached cells in tissue culture plates and on microcarriers. | lld:pubmed |
| pubmed-article:2479296 | pubmed:affiliation | Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Brazil. | lld:pubmed |
| pubmed-article:2479296 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2479296 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
