| pubmed-article:2479292 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2479292 | lifeskim:mentions | umls-concept:C0020205 | lld:lifeskim |
| pubmed-article:2479292 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:2479292 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:2479292 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:2479292 | lifeskim:mentions | umls-concept:C0086231 | lld:lifeskim |
| pubmed-article:2479292 | lifeskim:mentions | umls-concept:C0765250 | lld:lifeskim |
| pubmed-article:2479292 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2479292 | pubmed:dateCreated | 1989-12-12 | lld:pubmed |
| pubmed-article:2479292 | pubmed:abstractText | A sensitive nonisotopic solution hybridization assay for detection of RNA is described and characterized using a pSP65 plasmid model system. The assay procedure is based on a hybridization reaction in solution between a biotinylated DNA probe and a target RNA. The biotin-labeled hybrids are captured on a microtiter plate coated with an antibody to biotin. Bound DNA-RNA hybrids are detected by an immunoreaction with an enzyme-labeled monoclonal antibody specifically directed against DNA-RNA heteropolymers and the hybrids are quantitatively measured with the addition of a fluorogenic substrate. Optimal conditions under which to perform the assay were hybridization time, 1000 min; temperature, 75 degrees C; probe concentration, 0.2 microgram/ml; extent of probe biotinylation, 6.7%; buffer stringency, 2x SSC. A bisulfite-modified DNA probe was compared to nick-translated probes synthesized with reporter groups of different lengths (bio-11-dUTP or bio-19-dUTP). All probes could detect 10 pg/ml of target RNA. The presence of nonhomologous DNA or RNA sequences reduced the sensitivity of RNA detection by one half-log to 32 pg/ml (1.6 pg/assay). | lld:pubmed |
| pubmed-article:2479292 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479292 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479292 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2479292 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479292 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2479292 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479292 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479292 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479292 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2479292 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2479292 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:2479292 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:2479292 | pubmed:author | pubmed-author:YolkenR HRH | lld:pubmed |
| pubmed-article:2479292 | pubmed:author | pubmed-author:ViscidiR PRP | lld:pubmed |
| pubmed-article:2479292 | pubmed:author | pubmed-author:CoutleeFF | lld:pubmed |
| pubmed-article:2479292 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2479292 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2479292 | pubmed:volume | 181 | lld:pubmed |
| pubmed-article:2479292 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2479292 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2479292 | pubmed:pagination | 153-62 | lld:pubmed |
| pubmed-article:2479292 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2479292 | pubmed:meshHeading | pubmed-meshheading:2479292-... | lld:pubmed |
| pubmed-article:2479292 | pubmed:meshHeading | pubmed-meshheading:2479292-... | lld:pubmed |
| pubmed-article:2479292 | pubmed:meshHeading | pubmed-meshheading:2479292-... | lld:pubmed |
| pubmed-article:2479292 | pubmed:meshHeading | pubmed-meshheading:2479292-... | lld:pubmed |
| pubmed-article:2479292 | pubmed:meshHeading | pubmed-meshheading:2479292-... | lld:pubmed |
| pubmed-article:2479292 | pubmed:meshHeading | pubmed-meshheading:2479292-... | lld:pubmed |
| pubmed-article:2479292 | pubmed:meshHeading | pubmed-meshheading:2479292-... | lld:pubmed |
| pubmed-article:2479292 | pubmed:meshHeading | pubmed-meshheading:2479292-... | lld:pubmed |
| pubmed-article:2479292 | pubmed:meshHeading | pubmed-meshheading:2479292-... | lld:pubmed |
| pubmed-article:2479292 | pubmed:meshHeading | pubmed-meshheading:2479292-... | lld:pubmed |
| pubmed-article:2479292 | pubmed:meshHeading | pubmed-meshheading:2479292-... | lld:pubmed |
| pubmed-article:2479292 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2479292 | pubmed:articleTitle | Nonisotopic detection of RNA in an enzyme immunoassay using a monoclonal antibody against DNA-RNA hybrids. | lld:pubmed |
| pubmed-article:2479292 | pubmed:affiliation | Eudowood Division of Infectious Diseases, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205. | lld:pubmed |
| pubmed-article:2479292 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2479292 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2479292 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2479292 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2479292 | lld:pubmed |
