| pubmed-article:2477469 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2477469 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:2477469 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:2477469 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2477469 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2477469 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:2477469 | lifeskim:mentions | umls-concept:C1709634 | lld:lifeskim |
| pubmed-article:2477469 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:2477469 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:2477469 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2477469 | pubmed:dateCreated | 1989-11-14 | lld:pubmed |
| pubmed-article:2477469 | pubmed:abstractText | Using recombinant DNA technology, a 15-kD interferon (IFN)-induced protein and its 17-kD precursor have been expressed in Escherichia coli to obtain sufficient quantities of each protein for the investigation of their biological roles. Both the 15-kD and 17-kD proteins have been purified to homogeneity and crystallized. The recombinant 15-kD protein has an identical reversed-phase HPLC elution profile to that of the native 15-kD protein purified from human cells. Furthermore, the recombinant 15-kD and 17-kD proteins have identical amino- and carboxy-terminal amino acid sequences to those predicted from the DNA sequence. The native and recombinant 15-kD proteins give identical tryptic peptide maps, and the recombinant 17-kD protein gives only one additional tryptic peptide. We conclude that the recombinant 17-kD and 15-kD proteins are identical to the 17-kD precursor and the 15-kD stable product synthesized in human cells in response to IFN stimulation. In addition, we have demonstrated that the recombinant 17-kD precursor protein can be converted to the 15-kD protein by cytoplasmic extracts of human cells. | lld:pubmed |
| pubmed-article:2477469 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2477469 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477469 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2477469 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477469 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477469 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477469 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477469 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2477469 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2477469 | pubmed:issn | 0197-8357 | lld:pubmed |
| pubmed-article:2477469 | pubmed:author | pubmed-author:LarsenBB | lld:pubmed |
| pubmed-article:2477469 | pubmed:author | pubmed-author:KnightEEJr | lld:pubmed |
| pubmed-article:2477469 | pubmed:author | pubmed-author:FaheyDD | lld:pubmed |
| pubmed-article:2477469 | pubmed:author | pubmed-author:CordovaBB | lld:pubmed |
| pubmed-article:2477469 | pubmed:author | pubmed-author:BlomstromDD | lld:pubmed |
| pubmed-article:2477469 | pubmed:author | pubmed-author:FelthamNN | lld:pubmed |
| pubmed-article:2477469 | pubmed:author | pubmed-author:HillmanMMJr | lld:pubmed |
| pubmed-article:2477469 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2477469 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:2477469 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2477469 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2477469 | pubmed:pagination | 493-507 | lld:pubmed |
| pubmed-article:2477469 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:2477469 | pubmed:meshHeading | pubmed-meshheading:2477469-... | lld:pubmed |
| pubmed-article:2477469 | pubmed:meshHeading | pubmed-meshheading:2477469-... | lld:pubmed |
| pubmed-article:2477469 | pubmed:meshHeading | pubmed-meshheading:2477469-... | lld:pubmed |
| pubmed-article:2477469 | pubmed:meshHeading | pubmed-meshheading:2477469-... | lld:pubmed |
| pubmed-article:2477469 | pubmed:meshHeading | pubmed-meshheading:2477469-... | lld:pubmed |
| pubmed-article:2477469 | pubmed:meshHeading | pubmed-meshheading:2477469-... | lld:pubmed |
| pubmed-article:2477469 | pubmed:meshHeading | pubmed-meshheading:2477469-... | lld:pubmed |
| pubmed-article:2477469 | pubmed:meshHeading | pubmed-meshheading:2477469-... | lld:pubmed |
| pubmed-article:2477469 | pubmed:meshHeading | pubmed-meshheading:2477469-... | lld:pubmed |
| pubmed-article:2477469 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2477469 | pubmed:articleTitle | A 15-kD interferon-induced protein and its 17-kD precursor: expression in Escherichia coli, purification, and characterization. | lld:pubmed |
| pubmed-article:2477469 | pubmed:affiliation | Central Research and Development Department, E.I. du Pont de Nemours and Company, Inc., Wilmington, DE 19880-0328. | lld:pubmed |
| pubmed-article:2477469 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:9636 | entrezgene:pubmed | pubmed-article:2477469 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2477469 | lld:pubmed |
