| pubmed-article:2477459 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C0021753 | lld:lifeskim |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C0439851 | lld:lifeskim |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:2477459 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
| pubmed-article:2477459 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2477459 | pubmed:dateCreated | 1989-11-13 | lld:pubmed |
| pubmed-article:2477459 | pubmed:abstractText | A monoclonal antibody (mAb) specific for human recombinant IL-1 beta (hu rIL-1 beta) was produced by immunizing BALB/c mice with hu rIL-1 beta purified with classical methods. This mAb recognizes an epitope within the highly hydrophylic fragment spanning amino acid 133-147. The affinity constant of this mAb towards IL-1 beta was determined by RIA. An affinity column was prepared by covalent binding of the mAb to Sepharose CL-4B. The column was capable of selectively binding hu rIL-1 beta produced in Escherichia coli directly from crude homogenates. The IL-1 beta protein yield was higher than 90% with a very good recovery of IL-1 beta biological activity. Moreover, the immunosorbent retained at least two thirds of its IL-1 beta-binding capacity after 20 cycles of purification. | lld:pubmed |
| pubmed-article:2477459 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2477459 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477459 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2477459 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477459 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477459 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477459 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477459 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2477459 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2477459 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:2477459 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:2477459 | pubmed:author | pubmed-author:RossiRR | lld:pubmed |
| pubmed-article:2477459 | pubmed:author | pubmed-author:BoraschiDD | lld:pubmed |
| pubmed-article:2477459 | pubmed:author | pubmed-author:CasagliM CMC | lld:pubmed |
| pubmed-article:2477459 | pubmed:author | pubmed-author:BorriM GMG | lld:pubmed |
| pubmed-article:2477459 | pubmed:author | pubmed-author:VolpiniGG | lld:pubmed |
| pubmed-article:2477459 | pubmed:author | pubmed-author:BaldariCC | lld:pubmed |
| pubmed-article:2477459 | pubmed:author | pubmed-author:NucciDD | lld:pubmed |
| pubmed-article:2477459 | pubmed:author | pubmed-author:BigioMM | lld:pubmed |
| pubmed-article:2477459 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2477459 | pubmed:day | 29 | lld:pubmed |
| pubmed-article:2477459 | pubmed:volume | 123 | lld:pubmed |
| pubmed-article:2477459 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2477459 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2477459 | pubmed:pagination | 1-8 | lld:pubmed |
| pubmed-article:2477459 | pubmed:dateRevised | 2003-11-14 | lld:pubmed |
| pubmed-article:2477459 | pubmed:meshHeading | pubmed-meshheading:2477459-... | lld:pubmed |
| pubmed-article:2477459 | pubmed:meshHeading | pubmed-meshheading:2477459-... | lld:pubmed |
| pubmed-article:2477459 | pubmed:meshHeading | pubmed-meshheading:2477459-... | lld:pubmed |
| pubmed-article:2477459 | pubmed:meshHeading | pubmed-meshheading:2477459-... | lld:pubmed |
| pubmed-article:2477459 | pubmed:meshHeading | pubmed-meshheading:2477459-... | lld:pubmed |
| pubmed-article:2477459 | pubmed:meshHeading | pubmed-meshheading:2477459-... | lld:pubmed |
| pubmed-article:2477459 | pubmed:meshHeading | pubmed-meshheading:2477459-... | lld:pubmed |
| pubmed-article:2477459 | pubmed:meshHeading | pubmed-meshheading:2477459-... | lld:pubmed |
| pubmed-article:2477459 | pubmed:meshHeading | pubmed-meshheading:2477459-... | lld:pubmed |
| pubmed-article:2477459 | pubmed:meshHeading | pubmed-meshheading:2477459-... | lld:pubmed |
| pubmed-article:2477459 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2477459 | pubmed:articleTitle | One-step immunoaffinity purification of bioactive human recombinant IL-1 beta with a monoclonal antibody directed to a well-exposed domain of the protein. | lld:pubmed |
| pubmed-article:2477459 | pubmed:affiliation | Sclavo Research Center, Siena, Italy. | lld:pubmed |
| pubmed-article:2477459 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2477459 | lld:pubmed |
