| pubmed-article:2474393 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2474393 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:2474393 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:2474393 | lifeskim:mentions | umls-concept:C0205210 | lld:lifeskim |
| pubmed-article:2474393 | lifeskim:mentions | umls-concept:C1519266 | lld:lifeskim |
| pubmed-article:2474393 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2474393 | pubmed:dateCreated | 1989-9-20 | lld:pubmed |
| pubmed-article:2474393 | pubmed:abstractText | A sandwich-binding protein assay to determine the concentration of hyaluronic acid (HA) in body fluids has been developed. In this method, a hyaluronic acid binding protein (HABP) was adsorbed to the surface of a solid phase, and HA bound to HABP on the solid phase was detected by biotin-conjugated HABP. The method could assay HA levels within 6 hours using precoated microwells with HABP. HA could be determined in the range of 2-500 micrograms/l by this method using 50 microliters of serum. Within-run precision (CV) was 5.2-10.2%. The specificity of HABP to HA was confirmed by the elimination of the reaction with treatment by hyaluronidase digestion. Serum HA levels (median; range) of patients with rheumatoid arthritis (34; 2-187 micrograms/l) were shown to be higher than those with osteoarthritis (1; 1-21 micrograms/ml) and healthy controls (2; 1-8 micrograms/ml). No correlation between levels of HA and rheumatoid factor was found. HA was demonstrated to be a potential diagnostic marker for rheumatoid arthritis, and this HABP assay could be useful for determination of HA in clinical laboratory tests. | lld:pubmed |
| pubmed-article:2474393 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2474393 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2474393 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2474393 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2474393 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2474393 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2474393 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2474393 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2474393 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2474393 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2474393 | pubmed:month | May | lld:pubmed |
| pubmed-article:2474393 | pubmed:issn | 0009-8981 | lld:pubmed |
| pubmed-article:2474393 | pubmed:author | pubmed-author:MatsuuraTT | lld:pubmed |
| pubmed-article:2474393 | pubmed:author | pubmed-author:YokoyamaM MMM | lld:pubmed |
| pubmed-article:2474393 | pubmed:author | pubmed-author:ShichijoSS | lld:pubmed |
| pubmed-article:2474393 | pubmed:author | pubmed-author:ChichibuKK | lld:pubmed |
| pubmed-article:2474393 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2474393 | pubmed:day | 31 | lld:pubmed |
| pubmed-article:2474393 | pubmed:volume | 181 | lld:pubmed |
| pubmed-article:2474393 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2474393 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2474393 | pubmed:pagination | 317-23 | lld:pubmed |
| pubmed-article:2474393 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:2474393 | pubmed:meshHeading | pubmed-meshheading:2474393-... | lld:pubmed |
| pubmed-article:2474393 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2474393 | pubmed:articleTitle | Assay of serum hyaluronic acid in clinical application. | lld:pubmed |
| pubmed-article:2474393 | pubmed:affiliation | Chugai Pharmaceutical Co. Ltd., Tokyo, Japan. | lld:pubmed |
| pubmed-article:2474393 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2474393 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2474393 | lld:pubmed |
