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pubmed-article:2472978pubmed:abstractText1. Rat hepatocytes were cultured for 2 days in Williams E medium containing 1 microM insulin and dexamethasone. 2. Production of five plasma proteins was determined by electroimmunoassay in the media, and amino acid uptake was measured by [alpha-14C]aminoisobutyric acid accumulation in hepatocytes. 3. Supernatants from rat peritoneal macrophages and IL-6/IFN-beta 2/BSF-2 obtained from four different laboratories similarly stimulated synthesis of fibrinogen, alpha 1-cysteine proteinase inhibitor and alpha 2-macroglobulin, as well as [14C]-accumulation in cultured hepatocytes. 4. It is concluded that IL-6 is the principal hepatocyte stimulating factor responsible for typical features of the acute phase response of liver cells.lld:pubmed
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pubmed-article:2472978pubmed:articleTitleDifferent preparations of natural and recombinant human interleukin-6 (IFN-beta 2, BSF-2) similarly stimulate acute phase protein synthesis and uptake of alpha-aminoisobutyric acid by cultured rat hepatocytes.lld:pubmed
pubmed-article:2472978pubmed:affiliationInstitute of Molecular Biology, Jagiellonian University, Krakow, Poland.lld:pubmed
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pubmed-article:2472978pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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