| pubmed-article:2469478 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2469478 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:2469478 | lifeskim:mentions | umls-concept:C0021665 | lld:lifeskim |
| pubmed-article:2469478 | lifeskim:mentions | umls-concept:C1707455 | lld:lifeskim |
| pubmed-article:2469478 | lifeskim:mentions | umls-concept:C0205266 | lld:lifeskim |
| pubmed-article:2469478 | lifeskim:mentions | umls-concept:C0205460 | lld:lifeskim |
| pubmed-article:2469478 | pubmed:issue | 2-3 | lld:pubmed |
| pubmed-article:2469478 | pubmed:dateCreated | 1989-6-15 | lld:pubmed |
| pubmed-article:2469478 | pubmed:abstractText | A truncated form of insulin-like growth factor 1 (IGF-1), which lacked the aminoterminal tripeptide Gly-Pro-Glu has been isolated from human fetal and adult brain. This truncated IGF-1 displayed more potent cross-reactivity and biological action on brain cells than IGF-1 isolated from human serum. We now present data on a recombinant DNA-derived truncated IGF-1 lacking the aminoterminal tripeptide. Recombinant truncated IGF-1 was 1.4-5-times more potent than recombinant and natural IGF-1 in displacing [125 I]IGF-1 from human fetal and adult brain and placenta membranes. These differences were slightly enhanced when truncated IGF-1 was used as radioligand. The relative potencies compared to insulin-like growth factor 2 (IGF-2) in displacing [125I]IGF-2 from rat liver membranes were recombinant truncated IGF-1, 0.3% and recombinant IGF-1, 0.2%. Recombinant truncated IGF-1 displayed 100-fold reduced affinity for the low molecular weight binding protein (IGF-BP) isolated from human amniotic fluid when compared to recombinant IGF-1. Likewise, the IGF-BP was 100-fold less potent in inhibiting the receptor binding of recombinant truncated IGF-1 than that of recombinant IGF-1. Recombinant truncated IGF-1 was 4-times more potent than recombinant and natural IGF-1 in stimulating DNA synthesis in fetal rat brain cells. This biological activity of recombinant truncated IGF-1 was not affected by the IGF-BP at concentrations which abolished the biological activity of recombinant IGF-1. The hypothesis that IGF-BP bound intact IGF-1 represents the endocrine form of IGF-1, whereas truncated IGF-1 represents the paracrine or autocrine form of IGF-1, is proposed. | lld:pubmed |
| pubmed-article:2469478 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2469478 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2469478 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2469478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2469478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2469478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2469478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2469478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2469478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2469478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2469478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2469478 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2469478 | pubmed:month | May | lld:pubmed |
| pubmed-article:2469478 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:2469478 | pubmed:author | pubmed-author:HallKK | lld:pubmed |
| pubmed-article:2469478 | pubmed:author | pubmed-author:ShawT JTJ | lld:pubmed |
| pubmed-article:2469478 | pubmed:author | pubmed-author:LakeMM | lld:pubmed |
| pubmed-article:2469478 | pubmed:author | pubmed-author:HartmanisMM | lld:pubmed |
| pubmed-article:2469478 | pubmed:author | pubmed-author:Carlsson-Skwi... | lld:pubmed |
| pubmed-article:2469478 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2469478 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:2469478 | pubmed:volume | 1011 | lld:pubmed |
| pubmed-article:2469478 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2469478 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2469478 | pubmed:pagination | 192-7 | lld:pubmed |
| pubmed-article:2469478 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:meshHeading | pubmed-meshheading:2469478-... | lld:pubmed |
| pubmed-article:2469478 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2469478 | pubmed:articleTitle | A comparison of the biological activity of the recombinant intact and truncated insulin-like growth factor 1 (IGF-1). | lld:pubmed |
| pubmed-article:2469478 | pubmed:affiliation | Karolinska Institute's Department of Pathology, Karolinska Hospital, Stockholm, Sweden. | lld:pubmed |
| pubmed-article:2469478 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2469478 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:2469478 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:2469478 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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