| pubmed-article:2464496 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2464496 | lifeskim:mentions | umls-concept:C0029266 | lld:lifeskim |
| pubmed-article:2464496 | lifeskim:mentions | umls-concept:C0003316 | lld:lifeskim |
| pubmed-article:2464496 | lifeskim:mentions | umls-concept:C0597551 | lld:lifeskim |
| pubmed-article:2464496 | lifeskim:mentions | umls-concept:C0596448 | lld:lifeskim |
| pubmed-article:2464496 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:2464496 | pubmed:dateCreated | 1989-3-14 | lld:pubmed |
| pubmed-article:2464496 | pubmed:abstractText | The immunogenicity of synthetic peptide dimers based on epitope sequences derived from the mycobacterial 65-kDa antigen and the foot and mouth disease virus (FMDV) VP1 protein was examined in inbred mice. The analysis was directed towards the potential helper role of a T cell stimulatory mycobacterial epitope (65-85) with respect to poorly immunogenic sites either from the same molecule (422-436) or from VP1 (141-160). The 65-85 repeat homodimer induced an antibody response in CBA/ca but not in C57BL/6 mice, both nonresponders to the 65-85 monomer, and amplified the antibody response in BALB/c, monomer-responder mice. Analysis of the immunogenicity of hybrid dimers in BALB/c mice showed that the orientation of peptides within the dimer is critical for the extent of the produced antibody response. Only the 422-436/65-85 but not the 65-85/422-436 induced antibodies binding to the 422-436 sequence which was nonimmunogenic when injected either as a monomer or dimer. Despite the striking difference in immunogenicity, both tested hybrid dimers reacted equally in the solid-phase immunoassay with antisera raised to 65-85-dimer or 422-436/65-85 peptides or with a monoclonal antibody to the 422-436 epitope. The described differences in antibody responsiveness also cannot be attributed merely to the extent of T cell stimulation since the proliferative responses were uniformly expressed for all relevant combinations of peptides. Antisera to 65-85 dimer and 422-436/65-85 hybrid also reacted with the native 65-kDa protein. Furthermore, the production of FMDV-neutralizing antibodies in response to the 141-160 (VP1-derived)/65-85 hybrid peptide in 141-160 nonresponder B10.D2 mice also confirmed the helper activity of the 65-85 epitope. Thus, combining heterologous peptides with the N-terminal of the mycobacterial 65-85 sequence may be generally applicable for the potentiation of peptide vaccines. | lld:pubmed |
| pubmed-article:2464496 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2464496 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2464496 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2464496 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2464496 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:2464496 | pubmed:issn | 0014-2980 | lld:pubmed |
| pubmed-article:2464496 | pubmed:author | pubmed-author:FrancisM JMJ | lld:pubmed |
| pubmed-article:2464496 | pubmed:author | pubmed-author:CoxJ HJH | lld:pubmed |
| pubmed-article:2464496 | pubmed:author | pubmed-author:YoungD BDB | lld:pubmed |
| pubmed-article:2464496 | pubmed:author | pubmed-author:IvanyiJJ | lld:pubmed |
| pubmed-article:2464496 | pubmed:author | pubmed-author:LambJ RJR | lld:pubmed |
| pubmed-article:2464496 | pubmed:author | pubmed-author:SyredA DAD | lld:pubmed |
| pubmed-article:2464496 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2464496 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:2464496 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2464496 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2464496 | pubmed:pagination | 2015-9 | lld:pubmed |
| pubmed-article:2464496 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:2464496 | pubmed:meshHeading | pubmed-meshheading:2464496-... | lld:pubmed |
| pubmed-article:2464496 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2464496 | pubmed:articleTitle | Orientation of epitopes influences the immunogenicity of synthetic peptide dimers. | lld:pubmed |
| pubmed-article:2464496 | pubmed:affiliation | MRC Tuberculosis and Related Infections Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London. | lld:pubmed |
| pubmed-article:2464496 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2464496 | pubmed:publicationType | In Vitro | lld:pubmed |
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