| pubmed-article:2458525 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C0439849 | lld:lifeskim |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C0521119 | lld:lifeskim |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C1383501 | lld:lifeskim |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C0178539 | lld:lifeskim |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C0300926 | lld:lifeskim |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C1819179 | lld:lifeskim |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C1996904 | lld:lifeskim |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C1509144 | lld:lifeskim |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C1880497 | lld:lifeskim |
| pubmed-article:2458525 | lifeskim:mentions | umls-concept:C0337051 | lld:lifeskim |
| pubmed-article:2458525 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:2458525 | pubmed:dateCreated | 1988-10-18 | lld:pubmed |
| pubmed-article:2458525 | pubmed:abstractText | Addition of GnRH to pituitary gonadotrophs preloaded with Quin 2 resulted in a rapid (approximately 8 s) mobilization of an ionomycin-sensitive intracellular Ca2+ pool. A second component of Ca2+ entry via voltage dependent channels contributed about 45% of the peak cytosolic free Ca2+ concentration ([Ca2+]i). Thereafter, influx of Ca2+ via voltage-sensitive and -insensitive channels is responsible for maintenance of elevated [Ca2+]i during the second phase of GnRH action. Addition of inositol 1,4,5-trisphosphate (IP3) to permeabilized pituitary cells resulted in a Ca2+ transient, released from a nonmitochondrial pool, which maintained ambient free Ca2+ concentration around 170 nM in an ATP-dependent mechanism. Successive stimulations of the cells with IP3 produced an attenuated response. Elevation of the gonadotroph [Ca2+]i by ionomycin, to levels equivalent to that induced by GnRH, resulted in LH release amounting to only 45% of the response to the neurohormone. Activation of the voltage-dependent Ca2+ channels by the dihydropyridine Ca2+-agonist [methyl 1,4-dihydro-2,6-dimethyl-3-nitro-4-(2-trifluoromethylphenyl)-pyridine- 5-carboxylate (BAYK8644)] stimulated LH release, 36% of the GnRH (100 nM) response being reached by 10(-8) M of the drug, both [Ca2+]i elevation and GnRH-induced LH release were inhibited similarly (40-50%) by the dihydropyridine Ca2+-antagonist nifedipine. The results indicate that peak [Ca2+]i induced by GnRH in pituitary gonadotrophs is derived mainly from ionomycin-sensitive cellular stores most likely via IP3 formation.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:2458525 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2458525 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2458525 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2458525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2458525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2458525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2458525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2458525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2458525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2458525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2458525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2458525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2458525 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2458525 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:2458525 | pubmed:issn | 0888-8809 | lld:pubmed |
| pubmed-article:2458525 | pubmed:author | pubmed-author:CapponiA MAM | lld:pubmed |
| pubmed-article:2458525 | pubmed:author | pubmed-author:NaorZZ | lld:pubmed |
| pubmed-article:2458525 | pubmed:author | pubmed-author:AyalonDD | lld:pubmed |
| pubmed-article:2458525 | pubmed:author | pubmed-author:LimorRR | lld:pubmed |
| pubmed-article:2458525 | pubmed:author | pubmed-author:RossierM FMF | lld:pubmed |
| pubmed-article:2458525 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2458525 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:2458525 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2458525 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2458525 | pubmed:pagination | 512-20 | lld:pubmed |
| pubmed-article:2458525 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:meshHeading | pubmed-meshheading:2458525-... | lld:pubmed |
| pubmed-article:2458525 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2458525 | pubmed:articleTitle | Gonadotropin-releasing hormone-induced rise in cytosolic free Ca2+ levels: mobilization of cellular and extracellular Ca2+ pools and relationship to gonadotropin secretion. | lld:pubmed |
| pubmed-article:2458525 | pubmed:affiliation | Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel. | lld:pubmed |
| pubmed-article:2458525 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2458525 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:2458525 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:2458525 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2458525 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2458525 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2458525 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2458525 | lld:pubmed |
