| pubmed-article:2456323 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2456323 | lifeskim:mentions | umls-concept:C0521447 | lld:lifeskim |
| pubmed-article:2456323 | lifeskim:mentions | umls-concept:C0684076 | lld:lifeskim |
| pubmed-article:2456323 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2456323 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:2456323 | lifeskim:mentions | umls-concept:C0302891 | lld:lifeskim |
| pubmed-article:2456323 | lifeskim:mentions | umls-concept:C0055782 | lld:lifeskim |
| pubmed-article:2456323 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2456323 | pubmed:dateCreated | 1988-8-29 | lld:pubmed |
| pubmed-article:2456323 | pubmed:abstractText | A rapid three step procedure is described for the purification of C protein from HeLa 40 S hnRNP particles. The procedure takes advantage of the salt resistant RNA binding of C protein, the size of the C protein-RNA complex, and the strong binding of C protein to an anion-exchange resin. Typically 120 micrograms of C protein is obtained from 4.0 X 10(9) cells with greater than 95% electrophoretic purity. Proteins C1 and C2 copurify in the ratio of 3.5 Cl to 1 C2. The purified C protein participates in hnRNP particle reconstitution and on this basis is judged to be native. The purified C protein binds to a gel filtration matrix at 0.5 M NaCl but at higher salt concentrations it elutes before the marker protein, apoferritin (Mr = 443,000). An abbreviated two step purification procedure utilizing anion-exchange chromatography is also described. This procedure results in relatively pure C protein, as well as a useful separation of the other hnRNP proteins. | lld:pubmed |
| pubmed-article:2456323 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2456323 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2456323 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2456323 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2456323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2456323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2456323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2456323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2456323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2456323 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2456323 | pubmed:month | May | lld:pubmed |
| pubmed-article:2456323 | pubmed:issn | 0165-022X | lld:pubmed |
| pubmed-article:2456323 | pubmed:author | pubmed-author:FriedmanD LDL | lld:pubmed |
| pubmed-article:2456323 | pubmed:author | pubmed-author:LeStourgeonW... | lld:pubmed |
| pubmed-article:2456323 | pubmed:author | pubmed-author:BarnettS FSF | lld:pubmed |
| pubmed-article:2456323 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2456323 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:2456323 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2456323 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2456323 | pubmed:pagination | 87-97 | lld:pubmed |
| pubmed-article:2456323 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2456323 | pubmed:meshHeading | pubmed-meshheading:2456323-... | lld:pubmed |
| pubmed-article:2456323 | pubmed:meshHeading | pubmed-meshheading:2456323-... | lld:pubmed |
| pubmed-article:2456323 | pubmed:meshHeading | pubmed-meshheading:2456323-... | lld:pubmed |
| pubmed-article:2456323 | pubmed:meshHeading | pubmed-meshheading:2456323-... | lld:pubmed |
| pubmed-article:2456323 | pubmed:meshHeading | pubmed-meshheading:2456323-... | lld:pubmed |
| pubmed-article:2456323 | pubmed:meshHeading | pubmed-meshheading:2456323-... | lld:pubmed |
| pubmed-article:2456323 | pubmed:meshHeading | pubmed-meshheading:2456323-... | lld:pubmed |
| pubmed-article:2456323 | pubmed:meshHeading | pubmed-meshheading:2456323-... | lld:pubmed |
| pubmed-article:2456323 | pubmed:meshHeading | pubmed-meshheading:2456323-... | lld:pubmed |
| pubmed-article:2456323 | pubmed:meshHeading | pubmed-meshheading:2456323-... | lld:pubmed |
| pubmed-article:2456323 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2456323 | pubmed:articleTitle | Rapid purification of native C protein from nuclear ribonucleoprotein particles. | lld:pubmed |
| pubmed-article:2456323 | pubmed:affiliation | Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235. | lld:pubmed |
| pubmed-article:2456323 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2456323 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2456323 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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