| pubmed-article:2448217 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2448217 | lifeskim:mentions | umls-concept:C0019163 | lld:lifeskim |
| pubmed-article:2448217 | lifeskim:mentions | umls-concept:C0521026 | lld:lifeskim |
| pubmed-article:2448217 | lifeskim:mentions | umls-concept:C0025252 | lld:lifeskim |
| pubmed-article:2448217 | lifeskim:mentions | umls-concept:C0042210 | lld:lifeskim |
| pubmed-article:2448217 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:2448217 | lifeskim:mentions | umls-concept:C0205197 | lld:lifeskim |
| pubmed-article:2448217 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2448217 | pubmed:dateCreated | 1988-3-7 | lld:pubmed |
| pubmed-article:2448217 | pubmed:abstractText | The entire surface protein of hepatitis B virus serotype ayw containing the preS (preS1+preS2) and S domains has been expressed in the yeast Saccharomyces cerevisiae. Yeast containing a recombinant plasmid utilizing a constitutive promoter did not express this gene successfully due to the toxicity of the protein. A plasmid using a regulatable promoter directed expression which initiated late in the exponential phase of growth and resulted in the accumulation of high intracellular levels of the complete surface protein. The purified polypeptide aggregates into a form which, although not comprised of typical 20 nm particles, displays antigenic determinants encoded by the preS1, preS2 and S domains. Immunization of rabbits elicited the formation of antibodies directed against all three domains. This candidate vaccine will be useful for studying the contributions to viral immunity of the host response to the preS1 and preS2 domains. | lld:pubmed |
| pubmed-article:2448217 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2448217 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2448217 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2448217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2448217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2448217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2448217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2448217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2448217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2448217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2448217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2448217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2448217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2448217 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2448217 | pubmed:issn | 0270-9139 | lld:pubmed |
| pubmed-article:2448217 | pubmed:author | pubmed-author:MillerW JWJ | lld:pubmed |
| pubmed-article:2448217 | pubmed:author | pubmed-author:YamazakiSS | lld:pubmed |
| pubmed-article:2448217 | pubmed:author | pubmed-author:BurkePP | lld:pubmed |
| pubmed-article:2448217 | pubmed:author | pubmed-author:DunnNN | lld:pubmed |
| pubmed-article:2448217 | pubmed:author | pubmed-author:KniskernP JPJ | lld:pubmed |
| pubmed-article:2448217 | pubmed:author | pubmed-author:EllisR WRW | lld:pubmed |
| pubmed-article:2448217 | pubmed:author | pubmed-author:HagopianAA | lld:pubmed |
| pubmed-article:2448217 | pubmed:author | pubmed-author:EminiE AEA | lld:pubmed |
| pubmed-article:2448217 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2448217 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:2448217 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2448217 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2448217 | pubmed:pagination | 82-7 | lld:pubmed |
| pubmed-article:2448217 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:meshHeading | pubmed-meshheading:2448217-... | lld:pubmed |
| pubmed-article:2448217 | pubmed:articleTitle | A candidate vaccine for hepatitis B containing the complete viral surface protein. | lld:pubmed |
| pubmed-article:2448217 | pubmed:affiliation | Department of Virus and Cell Biology, Merck Sharp & Dohme Research Laboratories, West Point, Pennsylvania 19486. | lld:pubmed |
| pubmed-article:2448217 | pubmed:publicationType | Journal Article | lld:pubmed |
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