| pubmed-article:2447800 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2447800 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
| pubmed-article:2447800 | lifeskim:mentions | umls-concept:C0022677 | lld:lifeskim |
| pubmed-article:2447800 | lifeskim:mentions | umls-concept:C0596156 | lld:lifeskim |
| pubmed-article:2447800 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:2447800 | lifeskim:mentions | umls-concept:C0439799 | lld:lifeskim |
| pubmed-article:2447800 | pubmed:issue | 1 Pt 2 | lld:pubmed |
| pubmed-article:2447800 | pubmed:dateCreated | 1988-2-25 | lld:pubmed |
| pubmed-article:2447800 | pubmed:abstractText | The basolateral membrane of the rabbit proximal convoluted tubule (PCT) is known to be largely permeable to K ions. The patch-clamp technique was used to investigate the molecular basis of this K permeability. At room temperature and with a high-K solution (127 mM) in both the bathing medium and the patch pipette, current jumps associated with an inward-rectifying channel could be detected in every active cell-attached experiment. When the K concentration in the pipette was changed from 200 to 5 mM KCl (NaCl replacement), the single-channel conductance for inward currents changed from 54 to 10 pS. The observed shift in the zero current potential measured as a function of the patch pipette K concentration could be fitted using the Goldman-Hodgkin-Katz equation with a permeability ratio PNa/PK = 0.06. The channel was found to be moderately voltage dependent (e-fold per 56 mV depolarization). For instance, the open-channel probability (Po) increased from 0.06 to 0.16 following a membrane depolarization from -50 to +50 mV. A time interval distribution analysis showed for the open state a dominant single time constant of 14 and 10 ms at 50 and -50 mV, respectively. Two time constants equal to 1 (flickering) and 26 ms at +50 mV and to 0.6 and 300 ms at -50 mV were obtained for the closed-state interval distribution. Based on this analysis, it was concluded that the decrease of Po at negative potentials was due more to the appearance of prolonged silent periods than from a change in the channel mean open time. | lld:pubmed |
| pubmed-article:2447800 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2447800 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2447800 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2447800 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2447800 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2447800 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2447800 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2447800 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:2447800 | pubmed:author | pubmed-author:SauvéRR | lld:pubmed |
| pubmed-article:2447800 | pubmed:author | pubmed-author:ParentLL | lld:pubmed |
| pubmed-article:2447800 | pubmed:author | pubmed-author:CardinalJJ | lld:pubmed |
| pubmed-article:2447800 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2447800 | pubmed:volume | 254 | lld:pubmed |
| pubmed-article:2447800 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2447800 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2447800 | pubmed:pagination | F105-13 | lld:pubmed |
| pubmed-article:2447800 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2447800 | pubmed:meshHeading | pubmed-meshheading:2447800-... | lld:pubmed |
| pubmed-article:2447800 | pubmed:meshHeading | pubmed-meshheading:2447800-... | lld:pubmed |
| pubmed-article:2447800 | pubmed:meshHeading | pubmed-meshheading:2447800-... | lld:pubmed |
| pubmed-article:2447800 | pubmed:meshHeading | pubmed-meshheading:2447800-... | lld:pubmed |
| pubmed-article:2447800 | pubmed:meshHeading | pubmed-meshheading:2447800-... | lld:pubmed |
| pubmed-article:2447800 | pubmed:meshHeading | pubmed-meshheading:2447800-... | lld:pubmed |
| pubmed-article:2447800 | pubmed:meshHeading | pubmed-meshheading:2447800-... | lld:pubmed |
| pubmed-article:2447800 | pubmed:meshHeading | pubmed-meshheading:2447800-... | lld:pubmed |
| pubmed-article:2447800 | pubmed:meshHeading | pubmed-meshheading:2447800-... | lld:pubmed |
| pubmed-article:2447800 | pubmed:meshHeading | pubmed-meshheading:2447800-... | lld:pubmed |
| pubmed-article:2447800 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2447800 | pubmed:articleTitle | Single-channel analysis of a K channel at basolateral membrane of rabbit proximal convoluted tubule. | lld:pubmed |
| pubmed-article:2447800 | pubmed:affiliation | Membrane Transport Research Group, University of Montreal, Quebec, Canada. | lld:pubmed |
| pubmed-article:2447800 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2447800 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2447800 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2447800 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2447800 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2447800 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2447800 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2447800 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2447800 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2447800 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2447800 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2447800 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2447800 | lld:pubmed |
