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| pubmed-article:2444259 | lifeskim:mentions | umls-concept:C0029144 | lld:lifeskim |
| pubmed-article:2444259 | lifeskim:mentions | umls-concept:C1622418 | lld:lifeskim |
| pubmed-article:2444259 | lifeskim:mentions | umls-concept:C0023779 | lld:lifeskim |
| pubmed-article:2444259 | lifeskim:mentions | umls-concept:C0025251 | lld:lifeskim |
| pubmed-article:2444259 | lifeskim:mentions | umls-concept:C1522602 | lld:lifeskim |
| pubmed-article:2444259 | lifeskim:mentions | umls-concept:C0069792 | lld:lifeskim |
| pubmed-article:2444259 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2444259 | pubmed:dateCreated | 1987-11-23 | lld:pubmed |
| pubmed-article:2444259 | pubmed:abstractText | Experiments with large unilamellar dioleoylphosphatidylcholine vesicles were carried out in order to study the effect of membrane potential on the fluorescence of Oxonol VI. A partition equilibrium of dye between membrane and water was found to exist with a partition coefficient gamma identical to c lipid/c water of about 19,000 (at zero voltage). In the presence of an inside-positive membrane potential, the negatively charged dye accumulates in the intravesicular aqueous space according to a Nernst equilibrium. This leads to an increased adsorption of dye to the inner lipid monolayer and to a concomitant increase of fluorescence. The fluorescence change can be calibrated as a function of transmembrane voltage by generating a potassium diffusion potential in the presence of valinomycin. The intrinsic fluorescence of the membrane-bound dye is not affected by voltage; the whole influence of voltage on the fluorescence results from voltage-dependent partitioning of the dye between water and membrane. The voltage dependence of the apparent partition coefficient can be quantitatively described by a three-capacitor model in which the dye is assumed to bind to adsorption planes located on the hydrocarbon side of the membrane/solution interface. Oxonol VI was found to be suitable for detecting changes of membrane potential associated with the activity of the (Na+ + K+)-ATPase in reconstituted vesicles. When ATP is added to the external medium, pump molecules with the ATP-binding side facing outward become activated; this results in a translocation of net positive charge towards the vesicle interior. Under this condition, fluorescence changes corresponding to (inside-positive) potentials of up to 150-200 mV are observed. After the build-up of the membrane potential, a quasi-stationary state is reached in which the pump current is compensated by a back-flow of charge through passive conductance pathways. | lld:pubmed |
| pubmed-article:2444259 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2444259 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2444259 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2444259 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2444259 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2444259 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:2444259 | pubmed:author | pubmed-author:ApellH JHJ | lld:pubmed |
| pubmed-article:2444259 | pubmed:author | pubmed-author:BerschBB | lld:pubmed |
| pubmed-article:2444259 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2444259 | pubmed:day | 16 | lld:pubmed |
| pubmed-article:2444259 | pubmed:volume | 903 | lld:pubmed |
| pubmed-article:2444259 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2444259 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2444259 | pubmed:pagination | 480-94 | lld:pubmed |
| pubmed-article:2444259 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:2444259 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:2444259 | pubmed:articleTitle | Oxonol VI as an optical indicator for membrane potentials in lipid vesicles. | lld:pubmed |
| pubmed-article:2444259 | pubmed:affiliation | Department of Biology, University of Konstanz, F.R.G. | lld:pubmed |
| pubmed-article:2444259 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2444259 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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