Linked Life Data

2438694

Source:http://linkedlifedata.com/resource/pubmed/id/2438694

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pubmed-article:2438694pubmed:abstractTextA class I gene distinct from HLA-A, -B, or -C was identified in a cosmid clone and transfected into mouse L cells. The gene, placed adjacent to the polyoma enhancer, produced a full-length class I mRNA and high levels of a 43-kDa protein in the cytoplasm. The surface expression of the gene product required its association with human beta 2-microglobulin. The protein was recognized by a xenoantiserum raised against a mixture of human B- and T-cell lines. The product was also serologically reactive with the HLA framework monoclonal antibodies. The complete nucleotide sequence of the gene was determined and a specific oligonucleotide probe was synthesized. This probe was used to identify a full-length mRNA transcript in a B-lymphoblastoid cell line (JY). The gene was mapped within a 190-kilobase Not I restriction fragment located in the telomeric portion of the human major histocompatibility complex. Distinct features of the gene include the structure of the promoter, the position of the translation initiation site, a frameshift mutation at the carboxyl terminus, the insertion of an Alu repeat element in the eighth exon, divergence in the derived amino acid sequence, and the lack of expression of the gene in some cells.lld:pubmed
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pubmed-article:2438694pubmed:articleTitleStructure, expression, and molecular mapping of a divergent member of the class I HLA gene family.lld:pubmed
pubmed-article:2438694pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2438694pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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