| pubmed-article:2435726 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2435726 | lifeskim:mentions | umls-concept:C1517163 | lld:lifeskim |
| pubmed-article:2435726 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:2435726 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:2435726 | lifeskim:mentions | umls-concept:C0001455 | lld:lifeskim |
| pubmed-article:2435726 | lifeskim:mentions | umls-concept:C0011777 | lld:lifeskim |
| pubmed-article:2435726 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:2435726 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:2435726 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:2435726 | lifeskim:mentions | umls-concept:C0038891 | lld:lifeskim |
| pubmed-article:2435726 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:2435726 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:2435726 | pubmed:dateCreated | 1987-5-15 | lld:pubmed |
| pubmed-article:2435726 | pubmed:abstractText | Synthesis of surfactant-associated glycoprotein of Mr = 30,000-35,000 (SAP-35) was induced in explant culture of human fetal lung obtained from 8 to 24 weeks of gestation. SAP-35 synthesis and content increased markedly during 1-5 days in organ culture in association with the morphologic maturation of Type II epithelial cells and the appearance of lamellar bodies. [35S] Methionine labeling of the explants and subsequent immunoprecipitation of SAP-35 demonstrated distinct high-mannose precursors and sialylated SAP-35 forms as early in culture as SAP-35 synthesis was detectable. The increase in SAP-35 synthesis was associated with increased SAP-35 RNA of 2.1 kilobases as assessed by hybridization assay with [32P]cDNA specific for human SAP-35. Specific SAP-35 RNA increased during organ culture and both SAP-35 content and SAP-35 RNA increased in the absence of exogenous hormones in 2% carbon-stripped fetal calf serum. SAP-35 content and synthesis was stimulated by 8-Br-cAMP. Addition of 100 microM 8-Br-cAMP, enhanced both the concentration of SAP-35 protein and the SAP-35 RNA as assessed by hybridization assay. In contrast, treatment of the explants with dexamethasone was associated with decreased SAP-35 protein synthesis, SAP-35 content, and decreased SAP-35 RNA levels compared to untreated explants. Inhibition by dexamethasone occurred at all gestational ages tested, was dose-dependent, and detectable within 24-48 h during organ culture. Dexamethasone significantly inhibited both basal and cAMP-induced SAP-35 synthesis. Induction of pulmonary surfactant protein (SAP-35) synthesis during organ culture of human fetal lung was associated with increased SAP-35 RNA. SAP-35 synthesis and SAP-35 RNA were inhibited by dexamethasone and enhanced cAMP. | lld:pubmed |
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| pubmed-article:2435726 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2435726 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2435726 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2435726 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2435726 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2435726 | pubmed:author | pubmed-author:PilotTT | lld:pubmed |
| pubmed-article:2435726 | pubmed:author | pubmed-author:ClarkJ CJC | lld:pubmed |
| pubmed-article:2435726 | pubmed:author | pubmed-author:WhitsettJ AJA | lld:pubmed |
| pubmed-article:2435726 | pubmed:author | pubmed-author:WeaverT ETE | lld:pubmed |
| pubmed-article:2435726 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2435726 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2435726 | pubmed:volume | 262 | lld:pubmed |
| pubmed-article:2435726 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2435726 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2435726 | pubmed:pagination | 5256-61 | lld:pubmed |
| pubmed-article:2435726 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:2435726 | pubmed:meshHeading | pubmed-meshheading:2435726-... | lld:pubmed |
| pubmed-article:2435726 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:2435726 | pubmed:articleTitle | Induction of surfactant protein in fetal lung. Effects of cAMP and dexamethasone on SAP-35 RNA and synthesis. | lld:pubmed |
| pubmed-article:2435726 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2435726 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2435726 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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