| pubmed-article:2434478 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2434478 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:2434478 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:2434478 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:2434478 | lifeskim:mentions | umls-concept:C1327616 | lld:lifeskim |
| pubmed-article:2434478 | lifeskim:mentions | umls-concept:C0041194 | lld:lifeskim |
| pubmed-article:2434478 | lifeskim:mentions | umls-concept:C0439064 | lld:lifeskim |
| pubmed-article:2434478 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2434478 | pubmed:dateCreated | 1987-3-30 | lld:pubmed |
| pubmed-article:2434478 | pubmed:abstractText | High resolution gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis, cell-free translation, and elastin-specific antibodies were used to identify three tropoelastin isoforms secreted by bovine tissue and cells. Tropoelastin isolated from nuchal ligament and from conditioned culture medium or cell-matrix extracts of ligament fibroblasts and auricular chondrocytes resolved as three distinct bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with molecular weights of approximately 67,500 (tropoelastin I), 65,000 (tropoelastin II), and 62,000 (tropoelastin III). Three tropoelastin polypeptides with molecular mass 2-3 kDa higher than their corresponding tissue forms were also evident in cell-free translation products of ligamentum nuchae RNA, suggesting that each tropoelastin species is encoded by a unique mRNA. The presence of cysteine in all three tropoelastin isoforms was demonstrated by the incorporation of [35S]cysteine into newly synthesized tropoelastin polypeptides and by immunoreactivity with an antibody raised against a synthetic peptide that defines the cysteine-containing carboxyl-terminal region of tropoelastin. Immunological co-localization of the carboxyl-terminal antibody with insoluble elastin in lung vasculature and parenchyma suggests that intact tropoelastin and not a processed form is incorporated into the elastin fiber. | lld:pubmed |
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| pubmed-article:2434478 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2434478 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2434478 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2434478 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2434478 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2434478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2434478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2434478 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2434478 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:2434478 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2434478 | pubmed:author | pubmed-author:RosenbloomJJ | lld:pubmed |
| pubmed-article:2434478 | pubmed:author | pubmed-author:MechamR PRP | lld:pubmed |
| pubmed-article:2434478 | pubmed:author | pubmed-author:ParksW CWC | lld:pubmed |
| pubmed-article:2434478 | pubmed:author | pubmed-author:CrouchE CEC | lld:pubmed |
| pubmed-article:2434478 | pubmed:author | pubmed-author:WrennD SDS | lld:pubmed |
| pubmed-article:2434478 | pubmed:author | pubmed-author:KucichUU | lld:pubmed |
| pubmed-article:2434478 | pubmed:author | pubmed-author:WhitehouseL... | lld:pubmed |
| pubmed-article:2434478 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2434478 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2434478 | pubmed:volume | 262 | lld:pubmed |
| pubmed-article:2434478 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2434478 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2434478 | pubmed:pagination | 2244-9 | lld:pubmed |
| pubmed-article:2434478 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2434478 | pubmed:meshHeading | pubmed-meshheading:2434478-... | lld:pubmed |
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| pubmed-article:2434478 | pubmed:meshHeading | pubmed-meshheading:2434478-... | lld:pubmed |
| pubmed-article:2434478 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:2434478 | pubmed:articleTitle | Identification of multiple tropoelastins secreted by bovine cells. | lld:pubmed |
| pubmed-article:2434478 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2434478 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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