pubmed-article:2414278 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:2414278 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
pubmed-article:2414278 | lifeskim:mentions | umls-concept:C0033414 | lld:lifeskim |
pubmed-article:2414278 | lifeskim:mentions | umls-concept:C0384551 | lld:lifeskim |
pubmed-article:2414278 | pubmed:issue | 24 | lld:pubmed |
pubmed-article:2414278 | pubmed:dateCreated | 1985-11-29 | lld:pubmed |
pubmed-article:2414278 | pubmed:abstractText | We demonstrate by agarose gel electrophoresis and DNase I footprinting that Xenopus transcription factor A promotes DNA reassociation. This ability of factor A is dependent upon the domain-like structure of the protein. Digestion of factor A by papain results in a protein fragment which promotes DNA reassociation whereas a smaller fragment obtained by trypsin digestion does not. Although factor A requires zinc for specific binding to the 5 S RNA gene, the metal is not required for single-stranded DNA binding or promotion of DNA reassociation by this protein. The factor A-dependent renaturation of the 5 S RNA gene from its individual 32P end-labeled strands results in the proper gene conformation as evidenced by the restoration of the DNase I footprint characteristic of the intragenic control region. Alterations in DNase I cleavage patterns induced by factor A on the individual 5 S DNA strands are distinct from those induced by the protein on the duplex 5 S RNA gene. The ability of factor A to promote DNA reassociation further defines the possible roles of this protein in the formation of active transcription complexes and their maintenance during repeated rounds of transcription. | lld:pubmed |
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pubmed-article:2414278 | pubmed:language | eng | lld:pubmed |
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pubmed-article:2414278 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:2414278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2414278 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:2414278 | pubmed:month | Oct | lld:pubmed |
pubmed-article:2414278 | pubmed:issn | 0021-9258 | lld:pubmed |
pubmed-article:2414278 | pubmed:author | pubmed-author:WuC WCW | lld:pubmed |
pubmed-article:2414278 | pubmed:author | pubmed-author:HazudaD JDJ | lld:pubmed |
pubmed-article:2414278 | pubmed:author | pubmed-author:HanasJ SJS | lld:pubmed |
pubmed-article:2414278 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:2414278 | pubmed:day | 25 | lld:pubmed |
pubmed-article:2414278 | pubmed:volume | 260 | lld:pubmed |
pubmed-article:2414278 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:2414278 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:2414278 | pubmed:pagination | 13316-20 | lld:pubmed |
pubmed-article:2414278 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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pubmed-article:2414278 | pubmed:year | 1985 | lld:pubmed |
pubmed-article:2414278 | pubmed:articleTitle | Xenopus transcription factor A promotes DNA reassociation. | lld:pubmed |
pubmed-article:2414278 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:2414278 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:2414278 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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