| pubmed-article:2408570 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2408570 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2408570 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:2408570 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:2408570 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:2408570 | lifeskim:mentions | umls-concept:C0003595 | lld:lifeskim |
| pubmed-article:2408570 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2408570 | pubmed:dateCreated | 1985-7-17 | lld:pubmed |
| pubmed-article:2408570 | pubmed:abstractText | The secretion of newly synthesized apolipoprotein E (apo E) by human monocyte-derived macrophages (HMD macrophages) was measured in the medium of cells which had been incubated for 24 h with or without either native or acetylated low-density lipoproteins (LDL or AcLDL, respectively), and subsequently with [35S]methionine in the presence of high-density lipoproteins (HDL, 350 micrograms/ml) for 24 h, by isolating the lipoprotein fraction by centrifugation for 48 h at a density adjusted with KBr to 1.21 g/ml (d = 1.21). The d less than 1.21 medium was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or precipitation with trichloroacetic acid (TCA). Fluorography of the gels demonstrated that the d less than 1.21 fraction of the medium contained one major labeled band, which migrated with an apparent molecular weight of approximately 35,000. Immunoprecipitation of the d less than 1.21 fraction showed that the labeled band was precipitated by anti-apo E, but not by anti-HDL. As the apo E band appeared to be the only labeled band in the d less than 1.21 fraction, the amount of apo E secreted by the cells was quantitated by scintillation counting of the TCA-precipitable radioactivity in the d less than 1.21 fraction as compared with that in the whole medium. The proportion of secreted apo E to the total secreted protein was similar whether the cells had been in culture for 3 or 16 days, but was increased if the cells had been incubated with LDL or AcLDL. The proportion of apo E of the secreted proteins was always more than 6% and was as much as 16% when the cells were preincubated with lipoproteins, suggesting that the increased cholesterol influx induced apo E secretion. | lld:pubmed |
| pubmed-article:2408570 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2408570 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408570 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2408570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2408570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408570 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2408570 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:2408570 | pubmed:issn | 0003-9861 | lld:pubmed |
| pubmed-article:2408570 | pubmed:author | pubmed-author:KaydenH JHJ | lld:pubmed |
| pubmed-article:2408570 | pubmed:author | pubmed-author:TraberM GMG | lld:pubmed |
| pubmed-article:2408570 | pubmed:author | pubmed-author:MaschioFF | lld:pubmed |
| pubmed-article:2408570 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2408570 | pubmed:volume | 239 | lld:pubmed |
| pubmed-article:2408570 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2408570 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2408570 | pubmed:pagination | 388-95 | lld:pubmed |
| pubmed-article:2408570 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:2408570 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2408570 | pubmed:articleTitle | The secretion of apolipoprotein E by human monocyte-derived macrophages. | lld:pubmed |
| pubmed-article:2408570 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2408570 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2408570 | lld:pubmed |
