| pubmed-article:2408566 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2408566 | lifeskim:mentions | umls-concept:C0004611 | lld:lifeskim |
| pubmed-article:2408566 | lifeskim:mentions | umls-concept:C0314883 | lld:lifeskim |
| pubmed-article:2408566 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:2408566 | lifeskim:mentions | umls-concept:C2587213 | lld:lifeskim |
| pubmed-article:2408566 | lifeskim:mentions | umls-concept:C1707513 | lld:lifeskim |
| pubmed-article:2408566 | lifeskim:mentions | umls-concept:C1514152 | lld:lifeskim |
| pubmed-article:2408566 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2408566 | pubmed:dateCreated | 1985-7-25 | lld:pubmed |
| pubmed-article:2408566 | pubmed:abstractText | A low-copy-number plasmid vector, pHY481, was constructed by combining a macrolide resistance gene of a Staphylococcus aureus plasmid with a cryptic plasmid found in a Bacillus brevis strain isolated from soil. The plasmid introduced into B. brevis 47, an extensively investigated protein-producing bacterium, was maintained very stably in the absence of selective antibiotics. A Bacillus megaterium alpha-amylase gene subcloned into pHY481 was retained much more stably in B. brevis 47 than one subcloned into a plasmid of S. aureus origin. B. brevis 47 mutants were also isolated in which the copy number of pHY481 was amplified about 10-fold. The copy number of pHY481 with the inserted amylase gene also increased in the mutants. As a result, a severalfold-higher amount of the enzyme was produced in the mutants compared with that produced in wild-type B. brevis 47. Thus, the plasmid vector constructed here and the copy-number mutants of B. brevis 47 are useful for cloning foreign genes and performing genetic engineering in the protein-producing bacterium. | lld:pubmed |
| pubmed-article:2408566 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2408566 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2408566 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2408566 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2408566 | pubmed:month | May | lld:pubmed |
| pubmed-article:2408566 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:2408566 | pubmed:author | pubmed-author:NakagawaKK | lld:pubmed |
| pubmed-article:2408566 | pubmed:author | pubmed-author:TsukagoshiNN | lld:pubmed |
| pubmed-article:2408566 | pubmed:author | pubmed-author:YamagataHH | lld:pubmed |
| pubmed-article:2408566 | pubmed:author | pubmed-author:UdakaSS | lld:pubmed |
| pubmed-article:2408566 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2408566 | pubmed:volume | 49 | lld:pubmed |
| pubmed-article:2408566 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2408566 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2408566 | pubmed:pagination | 1076-9 | lld:pubmed |
| pubmed-article:2408566 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:2408566 | pubmed:meshHeading | pubmed-meshheading:2408566-... | lld:pubmed |
| pubmed-article:2408566 | pubmed:meshHeading | pubmed-meshheading:2408566-... | lld:pubmed |
| pubmed-article:2408566 | pubmed:meshHeading | pubmed-meshheading:2408566-... | lld:pubmed |
| pubmed-article:2408566 | pubmed:meshHeading | pubmed-meshheading:2408566-... | lld:pubmed |
| pubmed-article:2408566 | pubmed:meshHeading | pubmed-meshheading:2408566-... | lld:pubmed |
| pubmed-article:2408566 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2408566 | pubmed:articleTitle | A stable plasmid vector and control of its copy number in Bacillus brevis 47, a protein-producing bacterium. | lld:pubmed |
| pubmed-article:2408566 | pubmed:publicationType | Journal Article | lld:pubmed |
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