Linked Life Data

2406590

Source:http://linkedlifedata.com/resource/pubmed/id/2406590

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:2406590rdf:typepubmed:Citationlld:pubmed
pubmed-article:2406590lifeskim:mentionsumls-concept:C0521066lld:lifeskim
pubmed-article:2406590lifeskim:mentionsumls-concept:C0033739lld:lifeskim
pubmed-article:2406590lifeskim:mentionsumls-concept:C0017428lld:lifeskim
pubmed-article:2406590lifeskim:mentionsumls-concept:C2717970lld:lifeskim
pubmed-article:2406590lifeskim:mentionsumls-concept:C0679058lld:lifeskim
pubmed-article:2406590lifeskim:mentionsumls-concept:C1521871lld:lifeskim
pubmed-article:2406590lifeskim:mentionsumls-concept:C1547699lld:lifeskim
pubmed-article:2406590lifeskim:mentionsumls-concept:C1561491lld:lifeskim
pubmed-article:2406590lifeskim:mentionsumls-concept:C2700640lld:lifeskim
pubmed-article:2406590lifeskim:mentionsumls-concept:C0684192lld:lifeskim
pubmed-article:2406590pubmed:issue1lld:pubmed
pubmed-article:2406590pubmed:dateCreated1990-3-29lld:pubmed
pubmed-article:2406590pubmed:databankReferencehttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2406590pubmed:databankReferencehttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2406590pubmed:databankReferencehttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2406590pubmed:abstractTextCysteine protease gene fragments from three protozoan parasites Trypanosoma cruzi, Trypanosoma brucei, and Entamoeba histolytica were amplified by the polymerase chain reaction (PCR) from genomic DNA using degenerate oligonucleotide primers. The primers used for the amplification were designed based upon amino acid sequences flanking the active site cysteine and asparagine residues that are conserved in the eukaryotic cysteine proteases analyzed to date. The amplified DNA fragments, representing approximately 70% of the coding regions of the cysteine protease genes, were subcloned and sequenced. Sequence analysis and alignment showed significant sequence similarity to other members of the eukaryotic cysteine protease family (45% identical to chicken cathepsin L) and conservation of the cysteine, histidine, and asparagine residues which form the catalytic triad. These gene fragments provide molecular probes for further analysis of the structure and function of these important metabolic enzymes.lld:pubmed
pubmed-article:2406590pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2406590pubmed:languageenglld:pubmed
pubmed-article:2406590pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2406590pubmed:citationSubsetIMlld:pubmed
pubmed-article:2406590pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2406590pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2406590pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2406590pubmed:statusMEDLINElld:pubmed
pubmed-article:2406590pubmed:monthFeblld:pubmed
pubmed-article:2406590pubmed:issn0166-6851lld:pubmed
pubmed-article:2406590pubmed:authorpubmed-author:McKerrowJ HJHlld:pubmed
pubmed-article:2406590pubmed:authorpubmed-author:CrainC RCRlld:pubmed
pubmed-article:2406590pubmed:authorpubmed-author:BouvierJJlld:pubmed
pubmed-article:2406590pubmed:authorpubmed-author:SakanariJ AJAlld:pubmed
pubmed-article:2406590pubmed:authorpubmed-author:EakinA EAElld:pubmed
pubmed-article:2406590pubmed:issnTypePrintlld:pubmed
pubmed-article:2406590pubmed:volume39lld:pubmed
pubmed-article:2406590pubmed:ownerNLMlld:pubmed
pubmed-article:2406590pubmed:authorsCompleteYlld:pubmed
pubmed-article:2406590pubmed:pagination1-8lld:pubmed
pubmed-article:2406590pubmed:dateRevised2009-11-19lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:meshHeadingpubmed-meshheading:2406590-...lld:pubmed
pubmed-article:2406590pubmed:year1990lld:pubmed
pubmed-article:2406590pubmed:articleTitleAmplification and sequencing of genomic DNA fragments encoding cysteine proteases from protozoan parasites.lld:pubmed
pubmed-article:2406590pubmed:affiliationDepartment of Pathology, University of California, San Francisco.lld:pubmed
pubmed-article:2406590pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2406590pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:2406590pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
pubmed-article:2406590pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:2406590lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:2406590lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:2406590lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:2406590lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:2406590lld:pubmed