| pubmed-article:2406568 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2406568 | lifeskim:mentions | umls-concept:C0079460 | lld:lifeskim |
| pubmed-article:2406568 | lifeskim:mentions | umls-concept:C0079904 | lld:lifeskim |
| pubmed-article:2406568 | lifeskim:mentions | umls-concept:C1336776 | lld:lifeskim |
| pubmed-article:2406568 | lifeskim:mentions | umls-concept:C1333104 | lld:lifeskim |
| pubmed-article:2406568 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:2406568 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2406568 | pubmed:dateCreated | 1990-3-26 | lld:pubmed |
| pubmed-article:2406568 | pubmed:abstractText | The expression of the gene encoding the granulocyte-macrophage colony-stimulating factor (GM-CSF) is induced upon activation of T cells with phytohemagglutinin and active phorbolester and upon expression of tax1, a transactivating protein of the human T-cell leukemia virus type I. The same agents induce transcription from the interleukin-2 receptor alpha-chain and interleukin-2 genes, depending on promoter elements that bind the inducible transcription factor NF-kappa B (or an NF-kappa B-like factor). We therefore tested the possibility that the GM-CSF gene is also regulated by a cognate motif for the NF-kappa B transcription factor. A recent functional analysis by Miyatake et al. (S. Miyatake, M. Seiki, M. Yoshida, and K. Arai, Mol. Cell. Biol. 8:5581-5587, 1988) described a short promoter region in the GM-CSF gene that conferred strong inducibility by T-cell-activating signals and tax1, but no NF-kappa B-binding motifs were identified. Using electrophoretic mobility shift assays, we showed binding of purified human NF-kappa B and of the NF-kappa B activated in Jurkat T cells to an oligonucleotide comprising the GM-CSF promoter element responsible for mediating responsiveness to T-cell-activating signals and tax1. As shown by a methylation interference analysis and oligonucleotide competition experiments, purified NF-kappa B binds at positions -82 to -91 (GGGAACTACC) of the GM-CSF promoter sequence with an affinity similar to that with which it binds to the biologically functional kappa B motif in the beta interferon promoter (GGGAAATTCC). Two kappa B-like motifs at positions -98 to -108 of the GM-CSF promoter were also recognized but with much lower affinities. Our data provide strong evidence that the expression of the GM-CSF gene following T-cell activation is controlled by binding of the NF-kappa B transcription factor to a high-affinity binding site in the GM-CSF promoter. | lld:pubmed |
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| pubmed-article:2406568 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2406568 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2406568 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2406568 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:2406568 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:2406568 | pubmed:author | pubmed-author:SchreckRR | lld:pubmed |
| pubmed-article:2406568 | pubmed:author | pubmed-author:BaeuerleP APA | lld:pubmed |
| pubmed-article:2406568 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2406568 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:2406568 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2406568 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2406568 | pubmed:pagination | 1281-6 | lld:pubmed |
| pubmed-article:2406568 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2406568 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2406568 | pubmed:articleTitle | NF-kappa B as inducible transcriptional activator of the granulocyte-macrophage colony-stimulating factor gene. | lld:pubmed |
| pubmed-article:2406568 | pubmed:affiliation | Laboratorium für molekulare Biologie Ludwig-Maximilians-Universität München, Federal Republic of Germany. | lld:pubmed |
| pubmed-article:2406568 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2406568 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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