2373994

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Subject	Predicate	Object	Context
pubmed-article:2373994	rdf:type	pubmed:Citation	lld:pubmed
pubmed-article:2373994	lifeskim:mentions	umls-concept:C0039194	lld:lifeskim
pubmed-article:2373994	lifeskim:mentions	umls-concept:C0011306	lld:lifeskim
pubmed-article:2373994	lifeskim:mentions	umls-concept:C0003320	lld:lifeskim
pubmed-article:2373994	lifeskim:mentions	umls-concept:C0033684	lld:lifeskim
pubmed-article:2373994	lifeskim:mentions	umls-concept:C1947910	lld:lifeskim
pubmed-article:2373994	lifeskim:mentions	umls-concept:C1533691	lld:lifeskim
pubmed-article:2373994	lifeskim:mentions	umls-concept:C0444498	lld:lifeskim
pubmed-article:2373994	pubmed:issue	2	lld:pubmed
pubmed-article:2373994	pubmed:dateCreated	1990-8-30	lld:pubmed
pubmed-article:2373994	pubmed:abstractText	T cells recognize peptides that are bound to MHC molecules on the surface of different types of antigen-presenting cells (APC). Antigen presentation most often is studied using T cells that have undergone priming in situ, or cell lines that have been chronically stimulated in vitro. The use of primed cells provides sufficient numbers of antigen-reactive lymphocytes for experimental study. A more complete understanding of immunogenicity, however, requires that one develop systems for studying the onset of a T cell response from unprimed lymphocytes, especially in situ. Here it is shown that mouse T cells can be reliably primed in situ using dendritic cells as APC. The dendritic cells were isolated from spleen, pulsed with protein antigens, and then administered to naive mice. Antigen-responsive T cells developed in the draining lymphoid tissue, and these T cells only recognized protein when presented on cells bearing the same MHC products as the original priming dendritic cells. In contrast, little or no priming was seen if antigen-pulsed spleen cells or peritoneal cells were injected. Since very small amounts of the foreign protein were visualized within endocytic vacuoles of antigen-pulsed dendritic cells, it is suggested that dendritic cells have a small but relevant vacuolar system for presenting antigens over a several day period in situ.	lld:pubmed
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pubmed-article:2373994	pubmed:language	eng	lld:pubmed
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pubmed-article:2373994	pubmed:status	MEDLINE	lld:pubmed
pubmed-article:2373994	pubmed:month	Aug	lld:pubmed
pubmed-article:2373994	pubmed:issn	0022-1007	lld:pubmed
pubmed-article:2373994	pubmed:author	pubmed-author:InabaKK	lld:pubmed
pubmed-article:2373994	pubmed:author	pubmed-author:SteinmanR MRM	lld:pubmed
pubmed-article:2373994	pubmed:author	pubmed-author:MetlayJ PJP	lld:pubmed
pubmed-article:2373994	pubmed:author	pubmed-author:CrowleyM TMT	lld:pubmed
pubmed-article:2373994	pubmed:issnType	Print	lld:pubmed
pubmed-article:2373994	pubmed:day	1	lld:pubmed
pubmed-article:2373994	pubmed:volume	172	lld:pubmed
pubmed-article:2373994	pubmed:owner	NLM	lld:pubmed
pubmed-article:2373994	pubmed:authorsComplete	Y	lld:pubmed
pubmed-article:2373994	pubmed:pagination	631-40	lld:pubmed
pubmed-article:2373994	pubmed:dateRevised	2009-11-18	lld:pubmed
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pubmed-article:2373994	pubmed:year	1990	lld:pubmed
pubmed-article:2373994	pubmed:articleTitle	Dendritic cells pulsed with protein antigens in vitro can prime antigen-specific, MHC-restricted T cells in situ.	lld:pubmed
pubmed-article:2373994	pubmed:affiliation	Rockefeller University, Irvington Institute, New York, New York 10021.	lld:pubmed
pubmed-article:2373994	pubmed:publicationType	Journal Article	lld:pubmed
pubmed-article:2373994	pubmed:publicationType	Research Support, U.S. Gov't, P.H.S.	lld:pubmed
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