| pubmed-article:2368470 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2368470 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2368470 | lifeskim:mentions | umls-concept:C0376618 | lld:lifeskim |
| pubmed-article:2368470 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:2368470 | lifeskim:mentions | umls-concept:C0205210 | lld:lifeskim |
| pubmed-article:2368470 | lifeskim:mentions | umls-concept:C2347946 | lld:lifeskim |
| pubmed-article:2368470 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2368470 | pubmed:dateCreated | 1990-8-16 | lld:pubmed |
| pubmed-article:2368470 | pubmed:abstractText | An optimized chromogenic assay for the detection of endotoxins in human blood is described. The assay comprises the removal of the inhibitory activity of plasma components by a dilution plus heating procedure, endotoxin-dependent activation of Limulus amebocyte lysate, and chromogenic measurement of the activated lysate. The assay has a detection limit of 3 ng endotoxin/L plasma. At the 10 ng/L level within-assay CV and between-assay CV of 14 and 21% were obtained respectively. In a prospective clinical trial including 473 consecutive febrile patients the assay has previously been demonstrated to have positive and negative predictive values of 48% and 99% for impending Gram-negative sepsis, respectively. In a similar study in 76 consecutive patients with Gram-negative infection of the urinary tract, these values were 73% and 95%, respectively. We conclude that this assay may provide the means to select those patients who are most likely to benefit from anti-endotoxin treatment. To facilitate endotoxin testing in other laboratories, a preliminary evaluation of a commercial endotoxin assay versus our own method was performed with 108 duplicate blood samples obtained from septic patients. With this assay detection limits of 2-3 ng endotoxin/L plasma could be obtained, as well as a good correlation (r = 0.94) and level of consensus to establish endotoxemia (93%) as compared to the house method. The commercial assay may therefore facilitate the introduction of endotoxin testing in other laboratories. | lld:pubmed |
| pubmed-article:2368470 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2368470 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2368470 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2368470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2368470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2368470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2368470 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2368470 | pubmed:issn | 0323-5637 | lld:pubmed |
| pubmed-article:2368470 | pubmed:author | pubmed-author:ten CateJ WJW | lld:pubmed |
| pubmed-article:2368470 | pubmed:author | pubmed-author:SandersG TGT | lld:pubmed |
| pubmed-article:2368470 | pubmed:author | pubmed-author:SturmCC | lld:pubmed |
| pubmed-article:2368470 | pubmed:author | pubmed-author:van... | lld:pubmed |
| pubmed-article:2368470 | pubmed:author | pubmed-author:GallesH MHM | lld:pubmed |
| pubmed-article:2368470 | pubmed:author | pubmed-author:WortelC HCH | lld:pubmed |
| pubmed-article:2368470 | pubmed:author | pubmed-author:LevelsJ HJH | lld:pubmed |
| pubmed-article:2368470 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2368470 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:2368470 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2368470 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2368470 | pubmed:pagination | 147-58 | lld:pubmed |
| pubmed-article:2368470 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:2368470 | pubmed:meshHeading | pubmed-meshheading:2368470-... | lld:pubmed |
| pubmed-article:2368470 | pubmed:meshHeading | pubmed-meshheading:2368470-... | lld:pubmed |
| pubmed-article:2368470 | pubmed:meshHeading | pubmed-meshheading:2368470-... | lld:pubmed |
| pubmed-article:2368470 | pubmed:meshHeading | pubmed-meshheading:2368470-... | lld:pubmed |
| pubmed-article:2368470 | pubmed:meshHeading | pubmed-meshheading:2368470-... | lld:pubmed |
| pubmed-article:2368470 | pubmed:meshHeading | pubmed-meshheading:2368470-... | lld:pubmed |
| pubmed-article:2368470 | pubmed:meshHeading | pubmed-meshheading:2368470-... | lld:pubmed |
| pubmed-article:2368470 | pubmed:meshHeading | pubmed-meshheading:2368470-... | lld:pubmed |
| pubmed-article:2368470 | pubmed:meshHeading | pubmed-meshheading:2368470-... | lld:pubmed |
| pubmed-article:2368470 | pubmed:meshHeading | pubmed-meshheading:2368470-... | lld:pubmed |
| pubmed-article:2368470 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2368470 | pubmed:articleTitle | Detection and clinical relevance of human endotoxemia. | lld:pubmed |
| pubmed-article:2368470 | pubmed:affiliation | Department of Clinical Chemistry, Academic Medical Center, Amsterdam, The Netherlands. | lld:pubmed |
| pubmed-article:2368470 | pubmed:publicationType | Journal Article | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2368470 | lld:pubmed |
