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pubmed-article:2365500pubmed:abstractTextMonoclonal antibody (MAb) K 112 was generated after a single intrasplenic immunization with a recurrent laryngeal squamous-cell carcinoma. The antibody detects a 43-kDa nuclear antigen, with a pI of 5.4, which is expressed only in cycling cells. Expression is typically seen in a granular pattern excluding the nucleoli. During mitosis the bulk of the antigen is diffusely distributed in the cytoplasm. Identical reactivity was observed for tissues or cells of all mammalian species tested. These data indicate that MAb K 112 recognizes a protein belonging to the class of cell-cycle-related nuclear antigen molecules.lld:pubmed
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pubmed-article:2365500pubmed:articleTitleIdentification of a 43-kDa nuclear antigen associated with proliferation by monoclonal antibody K 112.lld:pubmed
pubmed-article:2365500pubmed:affiliationDept. of Otolaryngology/Head and Neck Surgery, Free University Hospital, Amsterdam, The Netherlands.lld:pubmed
pubmed-article:2365500pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2365500pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed