| pubmed-article:2335503 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2335503 | lifeskim:mentions | umls-concept:C0007603 | lld:lifeskim |
| pubmed-article:2335503 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:2335503 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:2335503 | lifeskim:mentions | umls-concept:C0017817 | lld:lifeskim |
| pubmed-article:2335503 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:2335503 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:2335503 | pubmed:dateCreated | 1990-6-13 | lld:pubmed |
| pubmed-article:2335503 | pubmed:abstractText | Transport of S-(2,4-dinitrophenyl)glutathione (DNP-SG), a model compound of glutathione S-conjugates, was studied in purified canalicular and basolateral (i.e. sinusoidal and lateral) rat liver plasma membrane (LPM) vesicles. Incubation of canalicular LPM vesicles with DNP-SG in the presence of ATP resulted in an uptake of DNP-SG into the vesicles. In contrast, in basolateral LPM vesicles the initial rate of ATP-dependent DNP-SG uptake was 3-4-fold lower than that of the canalicular LPM. This suggests that the ATP-dependent glutathione S-conjugates transport is localized in the canalicular membrane of the hepatocytes. The rate of DNP-SG uptake into canalicular LPM vesicles exhibited saturation kinetics with apparent Km values of 4 microM for DNP-SG and 260 microM for ATP. The DNP-SG uptake was inhibited by various glutathione S-conjugates and GSSG, not GSH. The bulkiness of S-substituent alkyl groups in the conjugates and cysteinylglycine of glutathione are essential for the inhibition. In contrast to the ATP-dependent process, the uptake of DNP-SG to basolateral LPM in the presence of KCl was 4-fold higher than that of canalicular LPM. The rate of DNP-SG uptake was enhanced by a valinomycin-induced K+ diffusion potential, and was inhibited by GSH. These results suggest that electrogenic transport of glutathione S-conjugates is localized in the basolateral membrane of the hepatocytes. These studies provide evidence that the ATP-dependent canalicular transport system functions in biliary secretion of glutathione S-conjugates. | lld:pubmed |
| pubmed-article:2335503 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2335503 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2335503 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2335503 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2335503 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2335503 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2335503 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2335503 | pubmed:month | May | lld:pubmed |
| pubmed-article:2335503 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2335503 | pubmed:author | pubmed-author:KobayashiKK | lld:pubmed |
| pubmed-article:2335503 | pubmed:author | pubmed-author:HayashiKK | lld:pubmed |
| pubmed-article:2335503 | pubmed:author | pubmed-author:HaraHH | lld:pubmed |
| pubmed-article:2335503 | pubmed:author | pubmed-author:SogameYY | lld:pubmed |
| pubmed-article:2335503 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2335503 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2335503 | pubmed:volume | 265 | lld:pubmed |
| pubmed-article:2335503 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2335503 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2335503 | pubmed:pagination | 7737-41 | lld:pubmed |
| pubmed-article:2335503 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:2335503 | pubmed:meshHeading | pubmed-meshheading:2335503-... | lld:pubmed |
| pubmed-article:2335503 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2335503 | pubmed:articleTitle | Mechanism of glutathione S-conjugate transport in canalicular and basolateral rat liver plasma membranes. | lld:pubmed |
| pubmed-article:2335503 | pubmed:affiliation | Institute of Scientific and Industrial Research, Osaka University, Japan. | lld:pubmed |
| pubmed-article:2335503 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2335503 | pubmed:publicationType | Comparative Study | lld:pubmed |
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