| pubmed-article:2324512 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2324512 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2324512 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
| pubmed-article:2324512 | lifeskim:mentions | umls-concept:C0684153 | lld:lifeskim |
| pubmed-article:2324512 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
| pubmed-article:2324512 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:2324512 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2324512 | pubmed:dateCreated | 1990-5-16 | lld:pubmed |
| pubmed-article:2324512 | pubmed:abstractText | We describe a new double-antibody ELISA system using avidin-biotin amplication for the mass measurement of mitochondrial aspartate aminotransferase (m-AST) in human serum. The assay is very sensitive and as little as 0.5 ng/ml of m-AST may be detected. The method is linear up to 100 micrograms/l. The within-day and day-to-day coefficients of variation were found to be 8.9% and 11.5% respectively for a low m-AST concentration (2.7 micrograms/l), and 5.9% and 8.0% respectively for a high level of m-AST (30 micrograms/l). The assay requires 100 microliters of serum and can be completed within 5 h. The ELISA procedure and a classical immunoprecipitation technique measuring the catalytic activity of the isoenzyme were applied simultaneously to the sera of 189 subjects. The protein levels determined by ELISA correlated poorly (r = 0.66) with the catalytic activity of m-AST. | lld:pubmed |
| pubmed-article:2324512 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2324512 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2324512 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2324512 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2324512 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2324512 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2324512 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2324512 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2324512 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2324512 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:2324512 | pubmed:author | pubmed-author:SiestGG | lld:pubmed |
| pubmed-article:2324512 | pubmed:author | pubmed-author:SchieleFF | lld:pubmed |
| pubmed-article:2324512 | pubmed:author | pubmed-author:WellmanMM | lld:pubmed |
| pubmed-article:2324512 | pubmed:author | pubmed-author:ArturYY | lld:pubmed |
| pubmed-article:2324512 | pubmed:author | pubmed-author:VarastehAA | lld:pubmed |
| pubmed-article:2324512 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2324512 | pubmed:day | 17 | lld:pubmed |
| pubmed-article:2324512 | pubmed:volume | 128 | lld:pubmed |
| pubmed-article:2324512 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2324512 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2324512 | pubmed:pagination | 203-9 | lld:pubmed |
| pubmed-article:2324512 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:meshHeading | pubmed-meshheading:2324512-... | lld:pubmed |
| pubmed-article:2324512 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2324512 | pubmed:articleTitle | An avidin-biotin ELISA for the measurement of mitochondrial aspartate aminotransferase in human serum. | lld:pubmed |
| pubmed-article:2324512 | pubmed:affiliation | Centre du Médicament, UA CNRS 597, Nancy, France. | lld:pubmed |
| pubmed-article:2324512 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2324512 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
