Injury induced by fatty acids or bile acid in isolated human colonocytes prevented by calcium.

Source:http://linkedlifedata.com/resource/pubmed/id/2322935

Cancer Lett. 1990 Apr 30 50 3 221-6

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Authors

Lipkin M, Friedman E, Galand P, Buset M, Winawer S

Affiliation

Hospital Erasme, Université Libre de Bruxelles, Belgium.

Abstract

Measurement of the modulation of the growth fraction of isolated normal colonocytes from adult subjects in primary monolayer culture was used as a sensitive quantitative assay to evaluate toxic effects of several endogenous compounds found within the colon. This assay was used to study the role of CaCl2 in blocking cell injury. When added simultaneously with the injurious agent, 5-10 mM CaCl2 blocked the toxicity of physiological concentrations of deoxycholic acid, oleic acid, palmitic acid and linoleic acid.

PMID
2322935

Publication types

Research Support, Non-U.S. Gov't