| pubmed-article:2321756 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2321756 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:2321756 | lifeskim:mentions | umls-concept:C0086972 | lld:lifeskim |
| pubmed-article:2321756 | lifeskim:mentions | umls-concept:C0030956 | lld:lifeskim |
| pubmed-article:2321756 | lifeskim:mentions | umls-concept:C0005290 | lld:lifeskim |
| pubmed-article:2321756 | lifeskim:mentions | umls-concept:C0443343 | lld:lifeskim |
| pubmed-article:2321756 | lifeskim:mentions | umls-concept:C0008565 | lld:lifeskim |
| pubmed-article:2321756 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2321756 | pubmed:dateCreated | 1990-5-2 | lld:pubmed |
| pubmed-article:2321756 | pubmed:abstractText | Methods for examining altered regions in unstable mutant proteins are described. The strategy is illustrated using assembly defective Chinese hamster beta-tubulin subunits that are rapidly degraded in the cell. These unstable proteins are metabolically labeled to high specific activity and isolated as spots on two-dimensional gels. Conditions for the generation of tryptic peptides from gel pieces containing beta-tubulin and their subsequent resolution by HPLC have been worked out. Through a combination of dual labeling with various tritiated amino acids and [35S]methionine as well as partial sequence analysis, the identification of several HPLC peaks with the known sequence of beta-tubulin has been accomplished. This technique should greatly aid attempts to map the sites of mutational alterations in beta-tubulin polypeptides, and the general strategy should be readily applicable to other mutant proteins. | lld:pubmed |
| pubmed-article:2321756 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2321756 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2321756 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2321756 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2321756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2321756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2321756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2321756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2321756 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2321756 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2321756 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:2321756 | pubmed:author | pubmed-author:CookRR | lld:pubmed |
| pubmed-article:2321756 | pubmed:author | pubmed-author:CabralFF | lld:pubmed |
| pubmed-article:2321756 | pubmed:author | pubmed-author:MinottiA MAM | lld:pubmed |
| pubmed-article:2321756 | pubmed:author | pubmed-author:LoebL MLM | lld:pubmed |
| pubmed-article:2321756 | pubmed:author | pubmed-author:BoggsB ABA | lld:pubmed |
| pubmed-article:2321756 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2321756 | pubmed:volume | 184 | lld:pubmed |
| pubmed-article:2321756 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2321756 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2321756 | pubmed:pagination | 28-34 | lld:pubmed |
| pubmed-article:2321756 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:meshHeading | pubmed-meshheading:2321756-... | lld:pubmed |
| pubmed-article:2321756 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2321756 | pubmed:articleTitle | Identification of methionine-containing tryptic peptides of unstable beta-tubulin separated by reverse-phase high-performance liquid chromatography. | lld:pubmed |
| pubmed-article:2321756 | pubmed:affiliation | Department of Pharmacology, University of Texas Medical School, Houston 77225. | lld:pubmed |
| pubmed-article:2321756 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2321756 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2321756 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
