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pubmed-article:2278087pubmed:abstractTextThree murine monoclonal antibodies (Mabs) were raised against a cytoplasmic antigen of the yeast phase of the pathogenic fungus Sporothrix schenckii using a modification of standard hybridoma technology incorporating the immunosuppressive drug cyclophosphamide. When tested for species-specificity within the pathogenic dimorphic fungi one of these Mabs (S5) showed little cross-reactivity by enzyme-linked immunosorbent assay and Western blot, though there was some recognition of Paracoccidioides brasiliensis antigen. This Mab recognized a 70-75 kDa molecule on reduced Western blots of S. schenckii antigen. The other two Mabs (S12 and S15) showed cross-reactivity with all dimorphic fungal antigens tested, though they appeared to recognize a molecule of similar molecular weight. This is the first report of any attempt to raise species-specific Mabs against this important causative agent of dermatological disease.lld:pubmed
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pubmed-article:2278087pubmed:articleTitlePreparation of murine monoclonal antibodies against the yeast phase of the dimorphic fungus Sporothrix schenckii.lld:pubmed
pubmed-article:2278087pubmed:affiliationDepartment of Clinical Sciences, Guy's Hospital, London Bridge, UK.lld:pubmed
pubmed-article:2278087pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2278087pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed