| pubmed-article:2273054 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2273054 | lifeskim:mentions | umls-concept:C1267092 | lld:lifeskim |
| pubmed-article:2273054 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:2273054 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:2273054 | lifeskim:mentions | umls-concept:C0002240 | lld:lifeskim |
| pubmed-article:2273054 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2273054 | pubmed:dateCreated | 1991-2-25 | lld:pubmed |
| pubmed-article:2273054 | pubmed:abstractText | We have previously shown that treatment of postconfluent, quiescent rat vascular smooth muscle cells (SMC) with platelet-derived growth factor (PDGF) dramatically reduced smooth muscle (SM) alpha-actin synthesis and SM alpha-actin mRNA abundance, suggesting a role for this mitogen in the control of SMC differentiation. In the present studies, we explored the molecular mechanisms whereby PDGF decreases SM alpha-actin mRNA levels. Treatment of postconfluent SMC with both platelet PDGF and recombinant PDGF-BB resulted in a dramatic and concentration-dependent decrease in SM alpha-actin mRNA levels. We observed no differences in efficacy between platelet PDGF and PDGF-BB, indicating that the PDGF-A chain is not required for the effect. The rate of decrease in SM alpha-actin mRNA abundance in PDGF-treated SMC was greater than that observed in cells treated with the transcriptional inhibitor, actinomycin D, with or without PDGF, indicating that PDGF induced a transcriptionally dependent destabilization of the cytosolic SM alpha-actin mRNA pool. This effect appeared selective for SM alpha-actin, in that there was no evidence of a similar change in non-muscle (NM) beta-actin mRNA stability following PDGF treatment. Results of nuclear run-on analyses showed no differences in SM alpha-actin transcription between PDGF- and vehicle-treated SMC at either 4 or 24 hours following treatment, demonstrating that decreases in transcription of the SM alpha-actin gene did not contribute to PDGF-induced changes in SM alpha-actin mRNA abundance. Results of these studies support a possible role for PDGF in regulation of SMC differentiation via a post-transcriptional control mechanism. | lld:pubmed |
| pubmed-article:2273054 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2273054 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2273054 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2273054 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2273054 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2273054 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2273054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2273054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2273054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2273054 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2273054 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:2273054 | pubmed:issn | 0021-9541 | lld:pubmed |
| pubmed-article:2273054 | pubmed:author | pubmed-author:OwensG KGK | lld:pubmed |
| pubmed-article:2273054 | pubmed:author | pubmed-author:BlankR SRS | lld:pubmed |
| pubmed-article:2273054 | pubmed:author | pubmed-author:CorjayM HMH | lld:pubmed |
| pubmed-article:2273054 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2273054 | pubmed:volume | 145 | lld:pubmed |
| pubmed-article:2273054 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2273054 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2273054 | pubmed:pagination | 391-7 | lld:pubmed |
| pubmed-article:2273054 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2273054 | pubmed:meshHeading | pubmed-meshheading:2273054-... | lld:pubmed |
| pubmed-article:2273054 | pubmed:meshHeading | pubmed-meshheading:2273054-... | lld:pubmed |
| pubmed-article:2273054 | pubmed:meshHeading | pubmed-meshheading:2273054-... | lld:pubmed |
| pubmed-article:2273054 | pubmed:meshHeading | pubmed-meshheading:2273054-... | lld:pubmed |
| pubmed-article:2273054 | pubmed:meshHeading | pubmed-meshheading:2273054-... | lld:pubmed |
| pubmed-article:2273054 | pubmed:meshHeading | pubmed-meshheading:2273054-... | lld:pubmed |
| pubmed-article:2273054 | pubmed:meshHeading | pubmed-meshheading:2273054-... | lld:pubmed |
| pubmed-article:2273054 | pubmed:meshHeading | pubmed-meshheading:2273054-... | lld:pubmed |
| pubmed-article:2273054 | pubmed:meshHeading | pubmed-meshheading:2273054-... | lld:pubmed |
| pubmed-article:2273054 | pubmed:meshHeading | pubmed-meshheading:2273054-... | lld:pubmed |
| pubmed-article:2273054 | pubmed:meshHeading | pubmed-meshheading:2273054-... | lld:pubmed |
| pubmed-article:2273054 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2273054 | pubmed:articleTitle | Platelet-derived growth factor-induced destabilization of smooth muscle alpha-actin mRNA. | lld:pubmed |
| pubmed-article:2273054 | pubmed:affiliation | Department of Physiology, University of Virginia School of Medicine, Charlottesville 22908. | lld:pubmed |
| pubmed-article:2273054 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2273054 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2273054 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2273054 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2273054 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2273054 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2273054 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2273054 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2273054 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2273054 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2273054 | lld:pubmed |
