pubmed-article:2264286 | pubmed:abstractText | Mononuclear cells (MNCs) were isolated from the endometrium of cattle by enzymatic digestion followed by centrifugation on a density gradient. Of the cells isolated, 32 +/- 3.2% rosetted AET-treated sheep red blood cells, 9 +/- 0.4% possessed surface membrane immunoglobulin, and 15 +/- 6.1% were esterase positive. The cells possessed the ability to proliferate in the presence of T cell mitogens and produced T cell growth factor (TCGF) after in vitro stimulation with Concanavalin A. Endometrial MNCs did not appear to suppress the blastogenesis of peripheral blood MNCs. | lld:pubmed |