| pubmed-article:2247164 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2247164 | lifeskim:mentions | umls-concept:C0004651 | lld:lifeskim |
| pubmed-article:2247164 | lifeskim:mentions | umls-concept:C1623051 | lld:lifeskim |
| pubmed-article:2247164 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:2247164 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:2247164 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
| pubmed-article:2247164 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
| pubmed-article:2247164 | lifeskim:mentions | umls-concept:C0439828 | lld:lifeskim |
| pubmed-article:2247164 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
| pubmed-article:2247164 | pubmed:issue | 6301 | lld:pubmed |
| pubmed-article:2247164 | pubmed:dateCreated | 1991-1-10 | lld:pubmed |
| pubmed-article:2247164 | pubmed:abstractText | New ways of making antibodies have recently been demonstrated using gene technology. Immunoglobulin variable (V) genes are amplified from hybridomas or B cells using the polymerase chain reaction, and cloned into expression vectors. Soluble antibody fragments secreted from bacteria are then screened for binding activities. Screening of V genes would, however, be revolutionized if they could be expressed on the surface of bacteriophage. Phage carrying V genes that encode binding activities could then be selected directly with antigen. Here we show that complete antibody V domains can be displayed on the surface of fd bacteriophage, that the phage bind specifically to antigen and that rare phage (one in a million) can be isolated after affinity chromatography. | lld:pubmed |
| pubmed-article:2247164 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2247164 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2247164 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2247164 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2247164 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2247164 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2247164 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2247164 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:2247164 | pubmed:issn | 0028-0836 | lld:pubmed |
| pubmed-article:2247164 | pubmed:author | pubmed-author:WinterGG | lld:pubmed |
| pubmed-article:2247164 | pubmed:author | pubmed-author:ChiswellD JDJ | lld:pubmed |
| pubmed-article:2247164 | pubmed:author | pubmed-author:GriffithsA... | lld:pubmed |
| pubmed-article:2247164 | pubmed:author | pubmed-author:McCaffertyJJ | lld:pubmed |
| pubmed-article:2247164 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2247164 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:2247164 | pubmed:volume | 348 | lld:pubmed |
| pubmed-article:2247164 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2247164 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2247164 | pubmed:pagination | 552-4 | lld:pubmed |
| pubmed-article:2247164 | pubmed:dateRevised | 2006-4-21 | lld:pubmed |
| pubmed-article:2247164 | pubmed:meshHeading | pubmed-meshheading:2247164-... | lld:pubmed |
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| pubmed-article:2247164 | pubmed:meshHeading | pubmed-meshheading:2247164-... | lld:pubmed |
| pubmed-article:2247164 | pubmed:meshHeading | pubmed-meshheading:2247164-... | lld:pubmed |
| pubmed-article:2247164 | pubmed:meshHeading | pubmed-meshheading:2247164-... | lld:pubmed |
| pubmed-article:2247164 | pubmed:meshHeading | pubmed-meshheading:2247164-... | lld:pubmed |
| pubmed-article:2247164 | pubmed:meshHeading | pubmed-meshheading:2247164-... | lld:pubmed |
| pubmed-article:2247164 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2247164 | pubmed:articleTitle | Phage antibodies: filamentous phage displaying antibody variable domains. | lld:pubmed |
| pubmed-article:2247164 | pubmed:affiliation | MRC Laboratory of Molecular Biology, Cambridge, UK. | lld:pubmed |
| pubmed-article:2247164 | pubmed:publicationType | Journal Article | lld:pubmed |
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