| pubmed-article:2222858 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2222858 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:2222858 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:2222858 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:2222858 | lifeskim:mentions | umls-concept:C0054868 | lld:lifeskim |
| pubmed-article:2222858 | lifeskim:mentions | umls-concept:C0162801 | lld:lifeskim |
| pubmed-article:2222858 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2222858 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:2222858 | pubmed:dateCreated | 1990-12-19 | lld:pubmed |
| pubmed-article:2222858 | pubmed:abstractText | Cathepsin G was purified by single-step cation-exchange chromatography from rat polymorphonuclear leukocytes, obtained from the peritoneal cavity after induction of a mild peritonitis. The 26 N-terminal amino acids were determined and showed 73% identity to those of human cathepsin G. Total amino-acid composition demonstrated a high degree of basic amino acids in accordance with its high affinity for the cationic-exchange gel medium. The protein was found to be a glycoprotein with a glucosamine content of 7.4% of the calculated Mr28,900. On SDS/polyacrylamide-gel electrophoresis the protein showed a Mr of 28,400. It migrated as two bands in a gradient SDS/polyacrylamide-gel indicating isoforms. The pH optimum for the proteinase was determined to be 8.0-8.5 using Suc-Ala-Ala-Pro-Phe-Nan as substrate (Suc = 3-carboxypropionyl; Nan = 4-nitroanilide). Km and Kcat/Km values for Suc-Ala-Ala-Pro-Phe-Nan were 0.86mM and 280M-1S-1 and for Suc-Phe-Leu-Phe-Nan 0.24mM and 3600M-1S-1, respectively. | lld:pubmed |
| pubmed-article:2222858 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2222858 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2222858 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2222858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2222858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2222858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2222858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2222858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2222858 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2222858 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:2222858 | pubmed:issn | 0177-3593 | lld:pubmed |
| pubmed-article:2222858 | pubmed:author | pubmed-author:OhlssonKK | lld:pubmed |
| pubmed-article:2222858 | pubmed:author | pubmed-author:BjörkPP | lld:pubmed |
| pubmed-article:2222858 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2222858 | pubmed:volume | 371 | lld:pubmed |
| pubmed-article:2222858 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2222858 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2222858 | pubmed:pagination | 595-601 | lld:pubmed |
| pubmed-article:2222858 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:meshHeading | pubmed-meshheading:2222858-... | lld:pubmed |
| pubmed-article:2222858 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2222858 | pubmed:articleTitle | Purification and N-terminal amino-acid sequence analysis of rat polymorphonuclear leukocyte cathepsin G. | lld:pubmed |
| pubmed-article:2222858 | pubmed:affiliation | Department of Surgical Pathophysiology, University of Lund, Malmö General Hospital. | lld:pubmed |
| pubmed-article:2222858 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2222858 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:2222858 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:290257 | entrezgene:pubmed | pubmed-article:2222858 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2222858 | lld:pubmed |
