| pubmed-article:2208119 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2208119 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:2208119 | lifeskim:mentions | umls-concept:C0008565 | lld:lifeskim |
| pubmed-article:2208119 | lifeskim:mentions | umls-concept:C0205195 | lld:lifeskim |
| pubmed-article:2208119 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:2208119 | pubmed:dateCreated | 1990-11-21 | lld:pubmed |
| pubmed-article:2208119 | pubmed:abstractText | A highly sensitive and specific assay for the detection of N7-methyl-2'-deoxyguanosine (N7methyldG) has been developed by combining high-performance liquid chromatography, 32P-postlabeling, and nucleotide chromatography. Separation of normal nucleotides and adducts by high-performance liquid chromatography and then combining a portion of 2'-deoxyguanosine to the N7methyldG allows for quantitation using an internal standard. The directly determined molar ratio is not subject to errors in digestion, variable ATP-specific activity, or assumptions in relative adduct-labeling efficiency. The detection limit was one N7methyldG adduct in 10(7) unmodified 2'-deoxyguanosine bases. N7methyldG adducts have been detected in 5 human lung samples in which O6-methyl-2'-deoxyguanosine adducts had been previously determined. The mean ratio of N7methyldG to O6-methyl-2'-deoxyguanosine was determined to be approximately 10. The current assay complements the high-performance liquid chromatography/32P-postlabeling assay for O6-methyl-2'-deoxyguanosine and increases the detection sensitivity of DNA methylated by exogenous alkylating agents. | lld:pubmed |
| pubmed-article:2208119 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2208119 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2208119 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2208119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2208119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2208119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2208119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2208119 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2208119 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2208119 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:2208119 | pubmed:author | pubmed-author:HarrisC CCC | lld:pubmed |
| pubmed-article:2208119 | pubmed:author | pubmed-author:WestonAA | lld:pubmed |
| pubmed-article:2208119 | pubmed:author | pubmed-author:WilsonV LVL | lld:pubmed |
| pubmed-article:2208119 | pubmed:author | pubmed-author:ShieldsP GPG | lld:pubmed |
| pubmed-article:2208119 | pubmed:author | pubmed-author:PoveyA CAC | lld:pubmed |
| pubmed-article:2208119 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2208119 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2208119 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:2208119 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2208119 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2208119 | pubmed:pagination | 6580-4 | lld:pubmed |
| pubmed-article:2208119 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:2208119 | pubmed:meshHeading | pubmed-meshheading:2208119-... | lld:pubmed |
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| pubmed-article:2208119 | pubmed:meshHeading | pubmed-meshheading:2208119-... | lld:pubmed |
| pubmed-article:2208119 | pubmed:meshHeading | pubmed-meshheading:2208119-... | lld:pubmed |
| pubmed-article:2208119 | pubmed:meshHeading | pubmed-meshheading:2208119-... | lld:pubmed |
| pubmed-article:2208119 | pubmed:meshHeading | pubmed-meshheading:2208119-... | lld:pubmed |
| pubmed-article:2208119 | pubmed:meshHeading | pubmed-meshheading:2208119-... | lld:pubmed |
| pubmed-article:2208119 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2208119 | pubmed:articleTitle | Combined high-performance liquid chromatography/32P-postlabeling assay of N7-methyldeoxyguanosine. | lld:pubmed |
| pubmed-article:2208119 | pubmed:affiliation | Laboratory of Human Carcinogenesis, National Cancer Institute, NIH, Bethesda, Maryland 20892. | lld:pubmed |
| pubmed-article:2208119 | pubmed:publicationType | Journal Article | lld:pubmed |
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