| pubmed-article:2207241 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2207241 | lifeskim:mentions | umls-concept:C0014653 | lld:lifeskim |
| pubmed-article:2207241 | lifeskim:mentions | umls-concept:C0019046 | lld:lifeskim |
| pubmed-article:2207241 | lifeskim:mentions | umls-concept:C0007018 | lld:lifeskim |
| pubmed-article:2207241 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:2207241 | lifeskim:mentions | umls-concept:C1149062 | lld:lifeskim |
| pubmed-article:2207241 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2207241 | pubmed:dateCreated | 1990-10-31 | lld:pubmed |
| pubmed-article:2207241 | pubmed:abstractText | We have measured the forward and reverse rates of the allosteric transition between R (relaxed) and T (tense) quaternary structures for oxyhemoglobin A from which a single oxygen molecule was removed in pH 7, phosphate buffer, using the method of modulated excitation (Ferrone, F.A., and J.J. Hopfield. 1976. Proc. Natl. Acad. Sci. USA. 73:4497-4501 and Ferrone, F.A., A.J. Martino, and S. Basak. 1985. Biophys. J. 48:269-282). Despite the low quantum yield, which necessitated large light levels and an associated temperature rise, the data was of superior quality to the equivalent experiment with CO as a ligand, permitting comparison between the allosteric behavior of hemoglobin with different ligands. Qualitatively, the T structure is favored more strongly in triligated oxyhemoglobin than triligated carboxyhemoglobin. The rates for the allosteric transition with oxygen bound were essentially temperature independent, whereas for CO both the R----T and T----R rates increased with temperature, having an activation energy of 2.2 and 2.8 kcal, respectively. The R----T rate was higher for O2 than for CO being 3 x 10(3) s-1 vs. 1.6 x 10(3) s-1 for HbCO at 25 degrees C. The T----R rate for HbO2 was only 2 x 10(3) s-1, vs 4.2 x 10(3) s-1 for HbCO, giving an equilibrium constant between the structures greater than unity (L3 = 1.5). The data suggest that there may be some allosteric inequality between the subunits, but do not require (or rule out) ligand binding heterogeneity. The ligand-dependent differences are compatible with stereochemical studies of HbCO and HbO2. However,the large population of T species with three oxygen molecules bound is much greater than predicted by precision equilibrium studies and a generalized Szabo-Karplus model (Lee, A. W., M. Karplus, C. Poyart, and E. Bursaux. 1988. Biochemistry.27:1285-1301) or by the allosteric model of DiCera (Di Cera, E., C. H. Robert, and S. J. Gill. 1987. Biochemistry.26:4003-4008). | lld:pubmed |
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| pubmed-article:2207241 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2207241 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2207241 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2207241 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2207241 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:2207241 | pubmed:issn | 0006-3495 | lld:pubmed |
| pubmed-article:2207241 | pubmed:author | pubmed-author:FerroneF AFA | lld:pubmed |
| pubmed-article:2207241 | pubmed:author | pubmed-author:MartinsA FAF | lld:pubmed |
| pubmed-article:2207241 | pubmed:author | pubmed-author:ZhangN QNQ | lld:pubmed |
| pubmed-article:2207241 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2207241 | pubmed:volume | 58 | lld:pubmed |
| pubmed-article:2207241 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2207241 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2207241 | pubmed:pagination | 333-40 | lld:pubmed |
| pubmed-article:2207241 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2207241 | pubmed:meshHeading | pubmed-meshheading:2207241-... | lld:pubmed |
| pubmed-article:2207241 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2207241 | pubmed:articleTitle | Allosteric kinetics and equilibria differ for carbon monoxide and oxygen binding to hemoglobin. | lld:pubmed |
| pubmed-article:2207241 | pubmed:affiliation | Department of Physics and Atmospheric Science, Drexel University, Philadelphia, Pennsylvania 19104. | lld:pubmed |
| pubmed-article:2207241 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2207241 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:2207241 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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