| pubmed-article:2188684 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2188684 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2188684 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:2188684 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:2188684 | lifeskim:mentions | umls-concept:C0072599 | lld:lifeskim |
| pubmed-article:2188684 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2188684 | lifeskim:mentions | umls-concept:C1442757 | lld:lifeskim |
| pubmed-article:2188684 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:2188684 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:2188684 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2188684 | pubmed:dateCreated | 1990-7-2 | lld:pubmed |
| pubmed-article:2188684 | pubmed:abstractText | The present communication documents attempts to produce the mature form of human surfactant-associated protein B (SP-B) by modification of the 5' and 3' regions of the cDNA and expression of the truncated cDNAs after insertion into the vector pKK223-3. The 5' end of a cDNA for human SP-B (1407 base pairs) was reconstructed through the ligation of synthetic oligonucleotides to an internal PstI site in the 5' region. This construction coded for the initiation of protein synthesis at a Met codon adjacent to a codon for the N-terminal Phe of the mature polypeptide. Variable amounts of the 3' end of the human SP-B cDNA were deleted with mung bean nuclease and exonuclease III. The resulting blunt-ended 3' fragments were then ligated to a synthetic oligonucleotide linker designed to create a stop codon. The modified 5' and 3' ends were ligated to a short PstI-BamHI fragment isolated from the SP-B cDNA and inserted into the expression vector pKK223-3. In vitro translation of sense mRNAs derived from the truncated SP-B cDNAs yielded oligopeptides of appropriate molecular weights, as indicated by urea - sodium dodecyl sulphate - polyacrylamide gel electrophoresis of either intact or immunoprecipitated reaction mixtures. Expression of SP-B in Escherichia coli was confirmed by Northern blot analysis for the mRNAs corresponding to the truncated cDNAs in appropriately transformed bacteria induced with the galactose analog isopropyl-beta-thiogalactoside. Western blot analysis using rabbit antisera prepared against bovine SP-B confirmed the presence of mature SP-B in lipid extracts of transformed E. coli, but the amounts were very small.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:2188684 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2188684 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2188684 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2188684 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2188684 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2188684 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2188684 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2188684 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2188684 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2188684 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:2188684 | pubmed:issn | 0829-8211 | lld:pubmed |
| pubmed-article:2188684 | pubmed:author | pubmed-author:FordCC | lld:pubmed |
| pubmed-article:2188684 | pubmed:author | pubmed-author:HammondG LGL | lld:pubmed |
| pubmed-article:2188684 | pubmed:author | pubmed-author:PossmayerFF | lld:pubmed |
| pubmed-article:2188684 | pubmed:author | pubmed-author:HardingP GPG | lld:pubmed |
| pubmed-article:2188684 | pubmed:author | pubmed-author:RichardsonCC | lld:pubmed |
| pubmed-article:2188684 | pubmed:author | pubmed-author:LaiY LYL | lld:pubmed |
| pubmed-article:2188684 | pubmed:author | pubmed-author:MathialaganNN | lld:pubmed |
| pubmed-article:2188684 | pubmed:author | pubmed-author:MackieGG | lld:pubmed |
| pubmed-article:2188684 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2188684 | pubmed:volume | 68 | lld:pubmed |
| pubmed-article:2188684 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2188684 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2188684 | pubmed:pagination | 559-66 | lld:pubmed |
| pubmed-article:2188684 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2188684 | pubmed:meshHeading | pubmed-meshheading:2188684-... | lld:pubmed |
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| pubmed-article:2188684 | pubmed:meshHeading | pubmed-meshheading:2188684-... | lld:pubmed |
| pubmed-article:2188684 | pubmed:meshHeading | pubmed-meshheading:2188684-... | lld:pubmed |
| pubmed-article:2188684 | pubmed:meshHeading | pubmed-meshheading:2188684-... | lld:pubmed |
| pubmed-article:2188684 | pubmed:meshHeading | pubmed-meshheading:2188684-... | lld:pubmed |
| pubmed-article:2188684 | pubmed:meshHeading | pubmed-meshheading:2188684-... | lld:pubmed |
| pubmed-article:2188684 | pubmed:meshHeading | pubmed-meshheading:2188684-... | lld:pubmed |
| pubmed-article:2188684 | pubmed:meshHeading | pubmed-meshheading:2188684-... | lld:pubmed |
| pubmed-article:2188684 | pubmed:meshHeading | pubmed-meshheading:2188684-... | lld:pubmed |
| pubmed-article:2188684 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2188684 | pubmed:articleTitle | Expression of mature pulmonary surfactant-associated protein B (SP-B) in Escherichia coli using truncated human SP-B cDNAs. | lld:pubmed |
| pubmed-article:2188684 | pubmed:affiliation | Department of Obstetrics and Gynaecology, University of Western Ontario, London, Canada. | lld:pubmed |
| pubmed-article:2188684 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2188684 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2188684 | lld:pubmed |
