| pubmed-article:21785684 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21785684 | lifeskim:mentions | umls-concept:C0006826 | lld:lifeskim |
| pubmed-article:21785684 | lifeskim:mentions | umls-concept:C0006141 | lld:lifeskim |
| pubmed-article:21785684 | lifeskim:mentions | umls-concept:C0205462 | lld:lifeskim |
| pubmed-article:21785684 | lifeskim:mentions | umls-concept:C0005516 | lld:lifeskim |
| pubmed-article:21785684 | lifeskim:mentions | umls-concept:C0205307 | lld:lifeskim |
| pubmed-article:21785684 | lifeskim:mentions | umls-concept:C0205183 | lld:lifeskim |
| pubmed-article:21785684 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:21785684 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:21785684 | pubmed:dateCreated | 2011-7-25 | lld:pubmed |
| pubmed-article:21785684 | pubmed:abstractText | To detect the molecular changes of malignancy in histologically normal breast (HNB) tissues, we recently developed a novel 117-gene-malignancy-signature. Here we report validation of our leading malignancy-risk-genes, topoisomerase-2-alpha (TOP2A), minichromosome-maintenance-protein-2 (MCM2) and "budding-uninhibited-by-benzimidazoles-1-homolog-beta" (BUB1B) at the protein level. Using our 117-gene malignancy-signature, we classified 18 fresh-frozen HNB tissues from 18 adult female breast cancer patients into HNB-tissues with low-grade (HNB-LGMA; N = 9) and high-grade molecular abnormality (HNB-HGMA; N = 9). Archival sections of additional HNB tissues from these patients, and invasive ductal carcinoma (IDC) tissues from six other patients were immunostained for these biomarkers. TOP2A/MCM2 expression was assessed as staining index (%) and BUB1B expression as H-scores (0-300). Increasing TOP2A, MCM2, and BUB1B protein expression from HNB-LGMA to HNB-HGMA tissues to IDCs validated our microarray-based molecular classification of HNB tissues by immunohistochemistry. We also demonstrated an increasing expression of TOP2A protein on an independent test set of HNB/benign/reductionmammoplasties, atypical-ductal-hyperplasia with and without synchronous breast cancer, DCIS and IDC tissues using a custom tissue microarray (TMA). In conclusion, TOP2A, MCM2, and BUB1B proteins are potential molecular biomarkers of malignancy in histologically normal and benign breast tissues. Larger-scale clinical validation studies are needed to further evaluate the clinical utility of these molecular biomarkers. | lld:pubmed |
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| pubmed-article:21785684 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21785684 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21785684 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:21785684 | pubmed:issn | 2042-003X | lld:pubmed |
| pubmed-article:21785684 | pubmed:author | pubmed-author:McCarthySusan... | lld:pubmed |
| pubmed-article:21785684 | pubmed:author | pubmed-author:YeatmanTimoth... | lld:pubmed |
| pubmed-article:21785684 | pubmed:author | pubmed-author:HarrisEleanor... | lld:pubmed |
| pubmed-article:21785684 | pubmed:author | pubmed-author:ChenDung-TsaD... | lld:pubmed |
| pubmed-article:21785684 | pubmed:author | pubmed-author:NasirAejazA | lld:pubmed |
| pubmed-article:21785684 | pubmed:author | pubmed-author:VenkataramuCh... | lld:pubmed |
| pubmed-article:21785684 | pubmed:author | pubmed-author:KhakpourNazan... | lld:pubmed |
| pubmed-article:21785684 | pubmed:author | pubmed-author:GruidlMikeM | lld:pubmed |
| pubmed-article:21785684 | pubmed:author | pubmed-author:McBrideHeyoun... | lld:pubmed |
| pubmed-article:21785684 | pubmed:author | pubmed-author:Henderson-Jac... | lld:pubmed |
| pubmed-article:21785684 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21785684 | pubmed:volume | 2011 | lld:pubmed |
| pubmed-article:21785684 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21785684 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21785684 | pubmed:pagination | 489064 | lld:pubmed |
| pubmed-article:21785684 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21785684 | pubmed:articleTitle | Novel molecular markers of malignancy in histologically normal and benign breast. | lld:pubmed |
| pubmed-article:21785684 | pubmed:affiliation | Department of Anatomic Pathology, Moffitt Cancer Center & Research Institute, Tampa, FL 33612, USA. | lld:pubmed |
| pubmed-article:21785684 | pubmed:publicationType | Journal Article | lld:pubmed |
