| pubmed-article:21780504 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21780504 | lifeskim:mentions | umls-concept:C0000961 | lld:lifeskim |
| pubmed-article:21780504 | lifeskim:mentions | umls-concept:C0035499 | lld:lifeskim |
| pubmed-article:21780504 | lifeskim:mentions | umls-concept:C0018440 | lld:lifeskim |
| pubmed-article:21780504 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:21780504 | pubmed:dateCreated | 2011-7-25 | lld:pubmed |
| pubmed-article:21780504 | pubmed:abstractText | We cloned an intronless, nuclear-encoded opsin gene from an EST library of Acetabularia acetabulum. Acetabularia rhodopsin I (ARI) encodes a protein of 246 amino acids with molecular weight of 27 kDa. ARI was reconstituted in the Xenopus oocyte expression system to characterize its electrophysiological properties utilizing the two-electrode voltage-clamping technique. Oocytes where ARI cRNA was injected displayed outward directed currents in response to light. The maximum action spectrum of ARI was detected at 520 nm green light. Light-stimulated ARI current amplitude was altered by the protons, but not by the other ions in recording solutions, suggesting that the algal rhodopsin is a light-stimulated proton pump. Typical proton-mediated outward current elicited by 520 nm light was characterized with two phases of non-inactivating outward current following initial transient current. Taken together, we here reported cloning of a novel Acetabularia opsin gene which was characterized to be a proton-pump stimulated by light. | lld:pubmed |
| pubmed-article:21780504 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21780504 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21780504 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21780504 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21780504 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21780504 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21780504 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21780504 | pubmed:month | May | lld:pubmed |
| pubmed-article:21780504 | pubmed:issn | 1533-4880 | lld:pubmed |
| pubmed-article:21780504 | pubmed:author | pubmed-author:LeeSang-SooSS | lld:pubmed |
| pubmed-article:21780504 | pubmed:author | pubmed-author:LeeJung-HaJH | lld:pubmed |
| pubmed-article:21780504 | pubmed:author | pubmed-author:JungKwang-Hwa... | lld:pubmed |
| pubmed-article:21780504 | pubmed:author | pubmed-author:KimSo YoungSY | lld:pubmed |
| pubmed-article:21780504 | pubmed:author | pubmed-author:KangHo-WonHW | lld:pubmed |
| pubmed-article:21780504 | pubmed:author | pubmed-author:ChoiAh ReumAR | lld:pubmed |
| pubmed-article:21780504 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:21780504 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:21780504 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21780504 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21780504 | pubmed:pagination | 4596-600 | lld:pubmed |
| pubmed-article:21780504 | pubmed:meshHeading | pubmed-meshheading:21780504... | lld:pubmed |
| pubmed-article:21780504 | pubmed:meshHeading | pubmed-meshheading:21780504... | lld:pubmed |
| pubmed-article:21780504 | pubmed:meshHeading | pubmed-meshheading:21780504... | lld:pubmed |
| pubmed-article:21780504 | pubmed:meshHeading | pubmed-meshheading:21780504... | lld:pubmed |
| pubmed-article:21780504 | pubmed:meshHeading | pubmed-meshheading:21780504... | lld:pubmed |
| pubmed-article:21780504 | pubmed:meshHeading | pubmed-meshheading:21780504... | lld:pubmed |
| pubmed-article:21780504 | pubmed:meshHeading | pubmed-meshheading:21780504... | lld:pubmed |
| pubmed-article:21780504 | pubmed:meshHeading | pubmed-meshheading:21780504... | lld:pubmed |
| pubmed-article:21780504 | pubmed:meshHeading | pubmed-meshheading:21780504... | lld:pubmed |
| pubmed-article:21780504 | pubmed:meshHeading | pubmed-meshheading:21780504... | lld:pubmed |
| pubmed-article:21780504 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21780504 | pubmed:articleTitle | Acetabularia rhodopsin I is a light-stimulated proton pump. | lld:pubmed |
| pubmed-article:21780504 | pubmed:affiliation | Department of Life Science, Sogang University, Seoul 121-742, Republic of Korea. | lld:pubmed |
| pubmed-article:21780504 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21780504 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
