| pubmed-article:2176219 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2176219 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:2176219 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2176219 | lifeskim:mentions | umls-concept:C0677626 | lld:lifeskim |
| pubmed-article:2176219 | lifeskim:mentions | umls-concept:C1415615 | lld:lifeskim |
| pubmed-article:2176219 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2176219 | lifeskim:mentions | umls-concept:C0443470 | lld:lifeskim |
| pubmed-article:2176219 | lifeskim:mentions | umls-concept:C0443199 | lld:lifeskim |
| pubmed-article:2176219 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:2176219 | pubmed:issue | 36 | lld:pubmed |
| pubmed-article:2176219 | pubmed:dateCreated | 1991-2-14 | lld:pubmed |
| pubmed-article:2176219 | pubmed:abstractText | The level of hepatic triglyceride lipase (H-TGL) synthesis and secretion was examined in response to changes in cholesterol biosynthesis in the human hepatoma cell line HepG2. Cells were first fed a lipoprotein-deficient serum-supplemented medium to eliminate exogenous cholesterol. Mevinolin, a 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase inhibitor, was then added at a concentration (37 microM) which inhibited cholesterol biosynthesis by greater than 85% and decreased total cell cholesterol from 36.1 to 27.4 micrograms/ml of cell protein. Mevinolin treatment caused a 4.9 +/- 0.8-fold increase in the amount of H-TGL activity secreted into the medium, a 1.8 +/- 0.4-fold rise in H-TGL-specific mRNA, and a concurrent 14-fold increase in HMG-CoA reductase mRNA. Addition of 1 mM mevalonic acid to normal or mevinolin-treated cells raised the cellular cholesterol content and decreased the amount of secreted H-TGL activity to levels below control values. Mevalonic acid also prevented mevinolin-induction of H-TGL and HMG-CoA reductase mRNA, suggesting a common regulatory step for H-TGL and HMG-CoA reductase. Exposure of cells to mevinolin and 25-hydroxycholesterol together resulted in a marked repression of HMG-CoA reductase mRNA levels, whereas these conditions further enhanced the secretion of H-TGL activity and the expression of H-TGL mRNA. These results demonstrate a differential role for 25-hydroxycholesterol in the regulation of H-TGL and HMG-CoA reductase expression. | lld:pubmed |
| pubmed-article:2176219 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2176219 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2176219 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2176219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2176219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2176219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2176219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2176219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2176219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2176219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2176219 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2176219 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:2176219 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2176219 | pubmed:author | pubmed-author:MartinG AGA | lld:pubmed |
| pubmed-article:2176219 | pubmed:author | pubmed-author:JacksonR LRL | lld:pubmed |
| pubmed-article:2176219 | pubmed:author | pubmed-author:FlanaganM AMA | lld:pubmed |
| pubmed-article:2176219 | pubmed:author | pubmed-author:BarnhartR LRL | lld:pubmed |
| pubmed-article:2176219 | pubmed:author | pubmed-author:BuschS JSJ | lld:pubmed |
| pubmed-article:2176219 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2176219 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:2176219 | pubmed:volume | 265 | lld:pubmed |
| pubmed-article:2176219 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2176219 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2176219 | pubmed:pagination | 22474-9 | lld:pubmed |
| pubmed-article:2176219 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2176219 | pubmed:meshHeading | pubmed-meshheading:2176219-... | lld:pubmed |
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| pubmed-article:2176219 | pubmed:meshHeading | pubmed-meshheading:2176219-... | lld:pubmed |
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| pubmed-article:2176219 | pubmed:meshHeading | pubmed-meshheading:2176219-... | lld:pubmed |
| pubmed-article:2176219 | pubmed:meshHeading | pubmed-meshheading:2176219-... | lld:pubmed |
| pubmed-article:2176219 | pubmed:meshHeading | pubmed-meshheading:2176219-... | lld:pubmed |
| pubmed-article:2176219 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2176219 | pubmed:articleTitle | Differential regulation of hepatic triglyceride lipase and 3-hydroxy-3-methylglutaryl-CoA reductase gene expression in a human hepatoma cell line, HepG2. | lld:pubmed |
| pubmed-article:2176219 | pubmed:affiliation | Merrell Dow Research Institute, Cincinnati, Ohio 45215. | lld:pubmed |
| pubmed-article:2176219 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2176219 | pubmed:publicationType | Comparative Study | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2176219 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2176219 | lld:pubmed |
