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pubmed-article:2175525pubmed:abstractTextWe have examined methods for detection of pseudorabies virus (PRV) latency in three groups of swine; naturally infected animals obtained from a field case; animals which have been experimentally infected with Becker or Iowa strains of PRV; and single reactors (single seropositive animals within PRV-free herds). In situ hybridization was shown to be more sensitive than explanation/co-cultivation for the detection of latent virus. Nervous tissues, in particular the trigeminal ganglia, were found to be the most reliable source for detecting latent PRV. The presence of latent PRV was not detected in lymphoid tissues examined.lld:pubmed
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pubmed-article:2175525pubmed:authorpubmed-author:BrownT MTMlld:pubmed
pubmed-article:2175525pubmed:authorpubmed-author:RuleB GBGlld:pubmed
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pubmed-article:2175525pubmed:pagination273-80lld:pubmed
pubmed-article:2175525pubmed:dateRevised2003-11-14lld:pubmed
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pubmed-article:2175525pubmed:year1990lld:pubmed
pubmed-article:2175525pubmed:articleTitleDetection of latent pseudorabies virus in swine using in situ hybridization.lld:pubmed
pubmed-article:2175525pubmed:affiliationDepartment of Veterinary Sciences, University of Nebraska, Lincoln 68583-0905.lld:pubmed
pubmed-article:2175525pubmed:publicationTypeJournal Articlelld:pubmed
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