| pubmed-article:2174971 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2174971 | lifeskim:mentions | umls-concept:C0040113 | lld:lifeskim |
| pubmed-article:2174971 | lifeskim:mentions | umls-concept:C0230445 | lld:lifeskim |
| pubmed-article:2174971 | lifeskim:mentions | umls-concept:C0011644 | lld:lifeskim |
| pubmed-article:2174971 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:2174971 | lifeskim:mentions | umls-concept:C0012920 | lld:lifeskim |
| pubmed-article:2174971 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:2174971 | lifeskim:mentions | umls-concept:C2346484 | lld:lifeskim |
| pubmed-article:2174971 | lifeskim:mentions | umls-concept:C1881189 | lld:lifeskim |
| pubmed-article:2174971 | lifeskim:mentions | umls-concept:C0037791 | lld:lifeskim |
| pubmed-article:2174971 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2174971 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:2174971 | pubmed:dateCreated | 1991-1-24 | lld:pubmed |
| pubmed-article:2174971 | pubmed:abstractText | A large scale purification of DNA topoisomerase I from calf thymus was obtained using the combinations of 62% ammonium sulfate precipitation, Biorex 70, hydroxylapatite and phenylagarose chromatography. Silver staining of the preparation separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed 2 bands of apparent molecular weight of 70 and 60 kDa. Immunoblotting with a serum of anti-Scl-70 specificity also recognized both proteins. Anti-Scl-70 antibodies were detected in 24/32 (75%) patients with diffuse scleroderma by ELISA and 28/32 (87%) had the ability to neutralize the topoisomerase I activity. All of 14 sera with anticentromere antibodies did not react with DNA topoisomerase I by both assays. The presence of anti-Scl-70 antibodies remained disease specific despite the increase in sensitivity of both assays. | lld:pubmed |
| pubmed-article:2174971 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2174971 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2174971 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2174971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2174971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2174971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2174971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2174971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2174971 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2174971 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2174971 | pubmed:issn | 0315-162X | lld:pubmed |
| pubmed-article:2174971 | pubmed:author | pubmed-author:HwangJJ | lld:pubmed |
| pubmed-article:2174971 | pubmed:author | pubmed-author:ELEYC WCW | lld:pubmed |
| pubmed-article:2174971 | pubmed:author | pubmed-author:TorrS JSJ | lld:pubmed |
| pubmed-article:2174971 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2174971 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:2174971 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2174971 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2174971 | pubmed:pagination | 1314-9 | lld:pubmed |
| pubmed-article:2174971 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2174971 | pubmed:meshHeading | pubmed-meshheading:2174971-... | lld:pubmed |
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| pubmed-article:2174971 | pubmed:meshHeading | pubmed-meshheading:2174971-... | lld:pubmed |
| pubmed-article:2174971 | pubmed:meshHeading | pubmed-meshheading:2174971-... | lld:pubmed |
| pubmed-article:2174971 | pubmed:meshHeading | pubmed-meshheading:2174971-... | lld:pubmed |
| pubmed-article:2174971 | pubmed:meshHeading | pubmed-meshheading:2174971-... | lld:pubmed |
| pubmed-article:2174971 | pubmed:meshHeading | pubmed-meshheading:2174971-... | lld:pubmed |
| pubmed-article:2174971 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2174971 | pubmed:articleTitle | Specificity of anti-Scl-70 antibodies in scleroderma: increased sensitivity of detection using purified DNA topoisomerase I from calf thymus. | lld:pubmed |
| pubmed-article:2174971 | pubmed:affiliation | Department of Internal Medicine, Chung Shan Medical and Dental College, Taichung, ROC. | lld:pubmed |
| pubmed-article:2174971 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2174971 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2174971 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2174971 | lld:pubmed |
