| pubmed-article:21740892 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21740892 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:21740892 | lifeskim:mentions | umls-concept:C0389071 | lld:lifeskim |
| pubmed-article:21740892 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:21740892 | lifeskim:mentions | umls-concept:C0242738 | lld:lifeskim |
| pubmed-article:21740892 | lifeskim:mentions | umls-concept:C2246761 | lld:lifeskim |
| pubmed-article:21740892 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:21740892 | pubmed:dateCreated | 2011-8-1 | lld:pubmed |
| pubmed-article:21740892 | pubmed:abstractText | A decrease in adiponectin secretion leads to the early stage of atherosclerosis. Discoidal high-density lipoproteins (HDL) accept the cholesterol that effluxes from cells expressing the ATP binding cassette transporter A1 (ABCA1) in the first step of reverse cholesterol transport (RCT). Recently, a new therapeutic strategy involving reconstituted (r)HDL has been shown to enhance RCT. Therefore, we hypothesized that adiponectin may increase the efflux associated with ABCA1 and also enhance rHDL-induced efflux in human embryonic kidney 293 (HEK293T) cells. We transfected adiponectin receptor 1 and 2 (AdipoR1 and AdipoR2) cDNA into cells. The transfected cells were labeled with [(3)H]cholesterol following cholesterol loading with or without adiponectin for 24h. The levels of cholesterol efflux were analyzed using a liquid scintillation counter. Treatment with adiponectin was associated with significantly higher levels of efflux in AdipoR1- and AdipoR2-transfected cells. Interestingly, rHDL-induced cholesterol efflux was enhanced in the presence of adiponectin. The down-regulation of adiponectin receptors using short-hairpin RNA decreased rHDL-induced cholesterol efflux with the down-regulation of ABCA1. In summary, adiponectin and its receptors increased cholesterol efflux and also enhanced rHDL-induced efflux at least partially through an ABCA1 pathway. These results suggest that adiponectin may enhance the RCT system and induce an anti-atherogenic effect. | lld:pubmed |
| pubmed-article:21740892 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21740892 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21740892 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21740892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21740892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21740892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21740892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21740892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21740892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21740892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21740892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21740892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21740892 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21740892 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:21740892 | pubmed:issn | 1090-2104 | lld:pubmed |
| pubmed-article:21740892 | pubmed:author | pubmed-author:KadowakiTakas... | lld:pubmed |
| pubmed-article:21740892 | pubmed:author | pubmed-author:YamauchiToshi... | lld:pubmed |
| pubmed-article:21740892 | pubmed:author | pubmed-author:KitajimaKenK | lld:pubmed |
| pubmed-article:21740892 | pubmed:author | pubmed-author:MiuraShin-ich... | lld:pubmed |
| pubmed-article:21740892 | pubmed:author | pubmed-author:SakuKeijiroK | lld:pubmed |
| pubmed-article:21740892 | pubmed:author | pubmed-author:RyeKerry-Anne... | lld:pubmed |
| pubmed-article:21740892 | pubmed:author | pubmed-author:UeharaYoshina... | lld:pubmed |
| pubmed-article:21740892 | pubmed:author | pubmed-author:KiyaYoshihiro... | lld:pubmed |
| pubmed-article:21740892 | pubmed:copyrightInfo | Copyright © 2011 Elsevier Inc. All rights reserved. | lld:pubmed |
| pubmed-article:21740892 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21740892 | pubmed:day | 29 | lld:pubmed |
| pubmed-article:21740892 | pubmed:volume | 411 | lld:pubmed |
| pubmed-article:21740892 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21740892 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21740892 | pubmed:pagination | 305-11 | lld:pubmed |
| pubmed-article:21740892 | pubmed:meshHeading | pubmed-meshheading:21740892... | lld:pubmed |
| pubmed-article:21740892 | pubmed:meshHeading | pubmed-meshheading:21740892... | lld:pubmed |
| pubmed-article:21740892 | pubmed:meshHeading | pubmed-meshheading:21740892... | lld:pubmed |
| pubmed-article:21740892 | pubmed:meshHeading | pubmed-meshheading:21740892... | lld:pubmed |
| pubmed-article:21740892 | pubmed:meshHeading | pubmed-meshheading:21740892... | lld:pubmed |
| pubmed-article:21740892 | pubmed:meshHeading | pubmed-meshheading:21740892... | lld:pubmed |
| pubmed-article:21740892 | pubmed:meshHeading | pubmed-meshheading:21740892... | lld:pubmed |
| pubmed-article:21740892 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21740892 | pubmed:articleTitle | Possibility of increasing cholesterol efflux by adiponectin and its receptors through the ATP binding cassette transporter A1 in HEK293T cells. | lld:pubmed |
| pubmed-article:21740892 | pubmed:affiliation | Department of Cardiology, Fukuoka University School of Medicine, Fukuoka, Japan. | lld:pubmed |
| pubmed-article:21740892 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21740892 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:19 | entrezgene:pubmed | pubmed-article:21740892 | lld:entrezgene |
| entrez-gene:9370 | entrezgene:pubmed | pubmed-article:21740892 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:21740892 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:21740892 | lld:entrezgene |
