Linked Life Data

21687858

Source:http://linkedlifedata.com/resource/pubmed/id/21687858

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:21687858rdf:typepubmed:Citationlld:pubmed
pubmed-article:21687858lifeskim:mentionsumls-concept:C0022688lld:lifeskim
pubmed-article:21687858lifeskim:mentionsumls-concept:C0004927lld:lifeskim
pubmed-article:21687858lifeskim:mentionsumls-concept:C1704922lld:lifeskim
pubmed-article:21687858lifeskim:mentionsumls-concept:C0600210lld:lifeskim
pubmed-article:21687858lifeskim:mentionsumls-concept:C1533691lld:lifeskim
pubmed-article:21687858lifeskim:mentionsumls-concept:C0936012lld:lifeskim
pubmed-article:21687858lifeskim:mentionsumls-concept:C0444498lld:lifeskim
pubmed-article:21687858lifeskim:mentionsumls-concept:C0205374lld:lifeskim
pubmed-article:21687858pubmed:issue7lld:pubmed
pubmed-article:21687858pubmed:dateCreated2011-7-5lld:pubmed
pubmed-article:21687858pubmed:abstractTextWe present a simple method for rapid and automatic characterization of lymphocyte migration from time-lapse fluorescence microscopy data. Time-lapse imaging of natural killer (NK) cells in vitro and in situ, both showed that individual cells transiently alter their migration behavior. Typically, NK cells showed periods of high motility, interrupted by transient periods of slow migration or almost complete arrests. Analysis of in vitro data showed that these periods frequently coincided with contacts with target cells, sometimes leading to target cell lysis. However, NK cells were also commonly observed to stop independently of contact with other cells. In order to objectively characterize the migration of NK cells, we implemented a simple method to discriminate when NK cells stop or have low motilities, have periods of directed migration or undergo random movement. This was achieved using a sliding window approach and evaluating the mean squared displacement (MSD) to assess the migration coefficient and MSD curvature along trajectories from individual NK cells over time. The method presented here can be used to quickly and quantitatively assess the dynamics of individual cells as well as heterogeneity within ensembles. Furthermore, it may also be used as a tool to automatically detect transient stops due to the formation of immune synapses, cell division or cell death. We show that this could be particularly useful for analysis of in situ time-lapse fluorescence imaging data where most cells, as well as the extracellular matrix, are usually unlabelled and thus invisible.lld:pubmed
pubmed-article:21687858pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21687858pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21687858pubmed:languageenglld:pubmed
pubmed-article:21687858pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21687858pubmed:citationSubsetIMlld:pubmed
pubmed-article:21687858pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:21687858pubmed:statusMEDLINElld:pubmed
pubmed-article:21687858pubmed:monthJullld:pubmed
pubmed-article:21687858pubmed:issn1757-9708lld:pubmed
pubmed-article:21687858pubmed:authorpubmed-author:CahalanMichae...lld:pubmed
pubmed-article:21687858pubmed:authorpubmed-author:BrismarHjalma...lld:pubmed
pubmed-article:21687858pubmed:authorpubmed-author:Vanherberghen...lld:pubmed
pubmed-article:21687858pubmed:authorpubmed-author:LindströmSara...lld:pubmed
pubmed-article:21687858pubmed:authorpubmed-author:KowalewskiJac...lld:pubmed
pubmed-article:21687858pubmed:authorpubmed-author:GarrodKym RKRlld:pubmed
pubmed-article:21687858pubmed:authorpubmed-author:Andersson-Sva...lld:pubmed
pubmed-article:21687858pubmed:authorpubmed-author:ÖnfeltBjörnBlld:pubmed
pubmed-article:21687858pubmed:authorpubmed-author:KhorshidiMoha...lld:pubmed
pubmed-article:21687858pubmed:copyrightInfoThis journal is © The Royal Society of Chemistry 2011lld:pubmed
pubmed-article:21687858pubmed:issnTypeElectroniclld:pubmed
pubmed-article:21687858pubmed:volume3lld:pubmed
pubmed-article:21687858pubmed:ownerNLMlld:pubmed
pubmed-article:21687858pubmed:authorsCompleteYlld:pubmed
pubmed-article:21687858pubmed:pagination770-8lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:meshHeadingpubmed-meshheading:21687858...lld:pubmed
pubmed-article:21687858pubmed:year2011lld:pubmed
pubmed-article:21687858pubmed:articleTitleAnalysis of transient migration behavior of natural killer cells imaged in situ and in vitro.lld:pubmed
pubmed-article:21687858pubmed:affiliationDepartment of Applied Physics, Cell Physics, The Royal Institute of Technology, SE-106 91 Stockholm, Sweden.lld:pubmed
pubmed-article:21687858pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:21687858pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
pubmed-article:21687858pubmed:publicationTypeResearch Support, N.I.H., Extramurallld:pubmed