| pubmed-article:2166750 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2166750 | lifeskim:mentions | umls-concept:C0376325 | lld:lifeskim |
| pubmed-article:2166750 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:2166750 | lifeskim:mentions | umls-concept:C0018909 | lld:lifeskim |
| pubmed-article:2166750 | lifeskim:mentions | umls-concept:C0679412 | lld:lifeskim |
| pubmed-article:2166750 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:2166750 | lifeskim:mentions | umls-concept:C0018904 | lld:lifeskim |
| pubmed-article:2166750 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2166750 | pubmed:dateCreated | 1990-9-14 | lld:pubmed |
| pubmed-article:2166750 | pubmed:abstractText | Hepatitis A virus (HAV) harvested from infected MRC-5 cells can hemagglutinate various species of erythrocytes at acid pH (Eckels et al., 1989). Further studies revealed that the majority of the hemagglutinin (HA) in MRC-5 and BS-C-1 cells was cell-associated. A simplified procedure for preparing HAV-HA consisted of collecting infected cells in phosphate-buffered saline followed by three cycles of freeze-thawing and sonication. The fluids were clarified and stored at 4 degrees C. The analysis of HA by rate-zonal sucrose gradient centrifugation indicated that the majority of HA co-migrated with infectious virus. Complete inactivation of infectious HAV with 0.03% beta-propiolactone (BPL) did not affect HA activity, while inactivation with 0.05% formalin caused a 16-fold reduction in titer. There was no difference in HAI antibody titers when BPL-treated HA was compared to untreated HA in the hemagglutination inhibition (HAI) test. | lld:pubmed |
| pubmed-article:2166750 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2166750 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2166750 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2166750 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2166750 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2166750 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2166750 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2166750 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:2166750 | pubmed:issn | 0166-0934 | lld:pubmed |
| pubmed-article:2166750 | pubmed:author | pubmed-author:EckelsK HKH | lld:pubmed |
| pubmed-article:2166750 | pubmed:author | pubmed-author:ANCAVV | lld:pubmed |
| pubmed-article:2166750 | pubmed:author | pubmed-author:MarchwickiR... | lld:pubmed |
| pubmed-article:2166750 | pubmed:author | pubmed-author:DuboisD RDR | lld:pubmed |
| pubmed-article:2166750 | pubmed:author | pubmed-author:TimchakR LRL | lld:pubmed |
| pubmed-article:2166750 | pubmed:author | pubmed-author:SummersP LPL | lld:pubmed |
| pubmed-article:2166750 | pubmed:author | pubmed-author:BarvirD ADA | lld:pubmed |
| pubmed-article:2166750 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2166750 | pubmed:volume | 28 | lld:pubmed |
| pubmed-article:2166750 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2166750 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2166750 | pubmed:pagination | 299-304 | lld:pubmed |
| pubmed-article:2166750 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:2166750 | pubmed:meshHeading | pubmed-meshheading:2166750-... | lld:pubmed |
| pubmed-article:2166750 | pubmed:meshHeading | pubmed-meshheading:2166750-... | lld:pubmed |
| pubmed-article:2166750 | pubmed:meshHeading | pubmed-meshheading:2166750-... | lld:pubmed |
| pubmed-article:2166750 | pubmed:meshHeading | pubmed-meshheading:2166750-... | lld:pubmed |
| pubmed-article:2166750 | pubmed:meshHeading | pubmed-meshheading:2166750-... | lld:pubmed |
| pubmed-article:2166750 | pubmed:meshHeading | pubmed-meshheading:2166750-... | lld:pubmed |
| pubmed-article:2166750 | pubmed:meshHeading | pubmed-meshheading:2166750-... | lld:pubmed |
| pubmed-article:2166750 | pubmed:meshHeading | pubmed-meshheading:2166750-... | lld:pubmed |
| pubmed-article:2166750 | pubmed:meshHeading | pubmed-meshheading:2166750-... | lld:pubmed |
| pubmed-article:2166750 | pubmed:meshHeading | pubmed-meshheading:2166750-... | lld:pubmed |
| pubmed-article:2166750 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2166750 | pubmed:articleTitle | Preparation of noninfectious hepatitis A virus hemagglutinin for detecting hemagglutination inhibition antibodies. | lld:pubmed |
| pubmed-article:2166750 | pubmed:affiliation | Division of Communicable Disease and Immunology, Walter Reed Army Institute of Research, Washington, DC 20307-5100. | lld:pubmed |
| pubmed-article:2166750 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2166750 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2166750 | lld:pubmed |
