| pubmed-article:2163622 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2163622 | lifeskim:mentions | umls-concept:C1257792 | lld:lifeskim |
| pubmed-article:2163622 | lifeskim:mentions | umls-concept:C0699900 | lld:lifeskim |
| pubmed-article:2163622 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:2163622 | lifeskim:mentions | umls-concept:C0027479 | lld:lifeskim |
| pubmed-article:2163622 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2163622 | pubmed:dateCreated | 1990-8-8 | lld:pubmed |
| pubmed-article:2163622 | pubmed:abstractText | The binding, internalization and degradation of 200 pM monoiodinated human atrial natriuretic factor-(99-126) (125I-hANF) by cultured bovine aortic endothelial cells (BAECs) were studied at 37 degrees C. 125I-hANF was rapidly cleared from the extracellular medium (t1/2 approximately 10 min), whereas preincubation of the cells in the presence of 20 mM-NH4Cl or 0.2 mM-chloroquine resulted in a significant inhibition of this process. The BAECs rapidly produce three major degradation products of 125I-hANF, namely [125I]iodotyrosine 126 (125I-Y), Arg125-[125I]iodotyrosine126 (125I-RY) and Phe124-Arg125-[125I]iodotyrosine126(125I-FRY), which were detected in the extracellular medium. NH4Cl and chloroquine acted to inhibit the generation of 125I-Y and 125I-RY, but not that of 125I-FRY. Furthermore, excess unlabelled hANF (300 nM) completely blocked the rapid production of 125I-Y and 125I-RY in the first 5 min, but only partially (49%) inhibited the generation of 125I-FRY. Thus, in contrast with our previous findings with cultured smooth-muscle cells [Johnson, Arik & Foster (1989) J. Biol. Chem. 264, 11637-11642], BAECs bind, internalize and rapidly degrade 125I-hANF, resulting in the release of 125I-Y and 125I-RY into the extracellular medium. Similarly to smooth-muscle cells, the BAECs generate 125I-FRY from 125I-hANF via an extracellular proteolytic event. The rapidity of the receptor-mediated process and its sensitivity to NH4Cl and chloroquine suggest that the 125I-hANF is proteolytically processed in the endosomes of BAECs and that its receptors cycle between the cell surface and intracellular stores. | lld:pubmed |
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| pubmed-article:2163622 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2163622 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2163622 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2163622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2163622 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2163622 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:2163622 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:2163622 | pubmed:author | pubmed-author:JohnsonG RGR | lld:pubmed |
| pubmed-article:2163622 | pubmed:author | pubmed-author:PittsB JBJ | lld:pubmed |
| pubmed-article:2163622 | pubmed:author | pubmed-author:ArieRR | lld:pubmed |
| pubmed-article:2163622 | pubmed:author | pubmed-author:FosterC JCJ | lld:pubmed |
| pubmed-article:2163622 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2163622 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2163622 | pubmed:volume | 268 | lld:pubmed |
| pubmed-article:2163622 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2163622 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2163622 | pubmed:pagination | 771-6 | lld:pubmed |
| pubmed-article:2163622 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2163622 | pubmed:meshHeading | pubmed-meshheading:2163622-... | lld:pubmed |
| pubmed-article:2163622 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2163622 | pubmed:articleTitle | Rapid receptor-mediated catabolism of 125I-atrial natriuretic factor by vascular endothelial cells. | lld:pubmed |
| pubmed-article:2163622 | pubmed:affiliation | Section of Cardiovascular Biochemistry, Schering-Plough Research, Bloomfield, NJ 07003. | lld:pubmed |
| pubmed-article:2163622 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2163622 | lld:pubmed |
