Source:http://linkedlifedata.com/resource/pubmed/id/2162905
J. Exp. Med. 1990 Jul 1 172 1 61-8
General Info
Affiliation
Dipartimento di Biochimica e Biotecnologie Mediche, II Facoltà di Medicina, Napoli, Italy.Abstract
The biological role of interleukin 6 (IL-6) molecules in human B cell tumorigenesis was studied by using an episomal expression vector, pHEBoSV-IL6, to introduce stably the human IL-6 gene into human Epstein Barr virus (EBV)-transformed B lymphoblasts. The gene was present in the IL-6-transfected cells in a high copy number and was efficiently expressed, resulting in the secretion of consistent levels of IL-6 molecules. The constitutive expression of the IL-6 gene led to an altered pattern of growth and to a malignant phenotype, as shown by clonogenicity in to an altered pattern of growth and to a malignant phenotype, as shown by clonogenicity in soft agar cultures and tumorigenicity in nude mice. These data suggest that the combined action of EBV, which exerts an immortalizing function, and of the growth-promoting activity of IL-6 molecules, can give rise to fully transformed B cell tumors in immunodeficient subjects.
PMID
2162905
Publication types
Research Support, Non-U.S. Gov't