| pubmed-article:2162105 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2162105 | lifeskim:mentions | umls-concept:C1623048 | lld:lifeskim |
| pubmed-article:2162105 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:2162105 | lifeskim:mentions | umls-concept:C0010853 | lld:lifeskim |
| pubmed-article:2162105 | lifeskim:mentions | umls-concept:C1880352 | lld:lifeskim |
| pubmed-article:2162105 | lifeskim:mentions | umls-concept:C1707798 | lld:lifeskim |
| pubmed-article:2162105 | lifeskim:mentions | umls-concept:C1705294 | lld:lifeskim |
| pubmed-article:2162105 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2162105 | pubmed:dateCreated | 1990-7-18 | lld:pubmed |
| pubmed-article:2162105 | pubmed:abstractText | The cytopathic effects of vesicular stomatitis virus (VSV) that result in the rounding of BHK21 cells have been studied. The results indicate that they are mediated by a sequential alteration in the distribution of the components of the cytoskeleton, an effect that requires the expression of the viral L protein. The constituents of the cytoskeleton of BHK21 cells were analyzed by fluorescence microscopy. Actin filaments were the first component to become disorganized, so that disassembly of stress fibers were detected 1 hr after infection. The distribution of microtubules and intermediate filaments was unchanged at 2 hr after infection; however, both these cytoskeletal elements exhibited an altered distribution at 3-4 hr after infection. Actinomycin D and cycloheximide did not cause the same effects as infection with VSV, suggesting that inhibition of host-cell gene expression was not responsible. However, viral gene expression was required, since cells infected with uv-irradiated VSV showed the same distribution of cytoskeletal constituents as mock-infected controls. Cells infected at 39.5 degrees (the nonpermissive temperature) with mutants of VSV temperature sensitive in the viral NS (ts G22), N(ts G41), M(ts 0 23), and G(ts 0 45) proteins showed the same changes in the cytoskeleton as those detected with wild-type virus. In contrast, cells infected with ts G11 (L-) showed the characteristic effect of VSV on the cytoskeleton when incubated at 34 degrees (the permissive temperature), but not when incubated at 39.5 degrees. The T-1026 R1 mutant of VSV, which has a much less dramatic effect on cell morphology than wild-type virus, also caused a less marked disruption of the cytoskeleton. | lld:pubmed |
| pubmed-article:2162105 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2162105 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2162105 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2162105 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2162105 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2162105 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2162105 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2162105 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2162105 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:2162105 | pubmed:issn | 0042-6822 | lld:pubmed |
| pubmed-article:2162105 | pubmed:author | pubmed-author:WidnellC CCC | lld:pubmed |
| pubmed-article:2162105 | pubmed:author | pubmed-author:YoungnerJ SJS | lld:pubmed |
| pubmed-article:2162105 | pubmed:author | pubmed-author:Whitaker-Dowl... | lld:pubmed |
| pubmed-article:2162105 | pubmed:author | pubmed-author:SimonK OKO | lld:pubmed |
| pubmed-article:2162105 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2162105 | pubmed:volume | 177 | lld:pubmed |
| pubmed-article:2162105 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2162105 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2162105 | pubmed:pagination | 289-97 | lld:pubmed |
| pubmed-article:2162105 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:2162105 | pubmed:meshHeading | pubmed-meshheading:2162105-... | lld:pubmed |
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| pubmed-article:2162105 | pubmed:meshHeading | pubmed-meshheading:2162105-... | lld:pubmed |
| pubmed-article:2162105 | pubmed:meshHeading | pubmed-meshheading:2162105-... | lld:pubmed |
| pubmed-article:2162105 | pubmed:meshHeading | pubmed-meshheading:2162105-... | lld:pubmed |
| pubmed-article:2162105 | pubmed:meshHeading | pubmed-meshheading:2162105-... | lld:pubmed |
| pubmed-article:2162105 | pubmed:meshHeading | pubmed-meshheading:2162105-... | lld:pubmed |
| pubmed-article:2162105 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2162105 | pubmed:articleTitle | Sequential disassembly of the cytoskeleton in BHK21 cells infected with vesicular stomatitis virus. | lld:pubmed |
| pubmed-article:2162105 | pubmed:affiliation | Department of Neurobiology, Anatomy, and Cell Science, University of Pittsburgh School of Medicine, Pennsylvania 15261. | lld:pubmed |
| pubmed-article:2162105 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2162105 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2162105 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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